Studies on carbohydrate-metabolising enzymes. Part XVII. The enzymic synthesis of some disaccharides containing α-D-glucosyl residues

By the action of an enzyme preparation from Tetrahymena pyriformis, a-D-glucosyl residues may be transferred from phenyl a-D-glucopyranoside to various carbohydrates. By this means, the following disaccharides have been prepared and characterised: 6-O-a-D-glucopyranosyl-D-galactose and -D-mannose, 4-O-a-D-glucopyranosyl-L-xylose, 1-O-a-D-glucopyranosylribitol, 1-O-a-D-glucopyranosylerythritol, 1-O-a-D-glucopyranosyl-D-mannitol, and a mixture of 1-O- and 6-O-a-D-glucopyranosyl-D-glucitol. These results show the preferential transfer of a-D-glucosyl residues to primary alcohol groups in carbohydrate acceptor substrates. © 1968.