Structure of cell wall and exocellular mannans from the yeast Hansenula holstii. I. Mannans produced in phosphate containing medium

G San Blas, W L Cunningham

    Research output: Contribution to journalArticle

    Abstract

    Cell wall mannan and exocellular phosphomannan from Hansenula holstii NCYC 560 were analyzed to determine their structures. Both preparations were composed of a mixture of several polysaccharides (molecular weight = 1x106) differing in degree of phosphorylation and in structural arrangement. The cell wall mannan was a mixture of two branched polysaccharides containing 1,2 and 1,3 linkages, one of the fractions having ß linkages in addition to the a linkages usually found in these polymers. The exocellular phosphomannan was composed of three polymers, all of them with an a 1,6 linked backbone to which a 1,2 and a 1,3 linked side chains of various lengths were attached. The cell wall mannan contained phosphodiester linkages as reported for mannans from other species. However, the highly phosphorylated exocellular mannan was a novel kind of polysaccharide having its phosphates in the monoester form. It is concluded that the exocellular phosphomannan was not a product of autolysis of the cell wall material but rather an independently synthesized material

    Original languageEnglish
    Pages (from-to)233-246
    Number of pages14
    JournalBiochimica et Biophysica Acta - Gene Structure and Expression
    Volume354
    Issue number2
    Publication statusPublished - 1974

    Fingerprint Dive into the research topics of 'Structure of cell wall and exocellular mannans from the yeast Hansenula holstii. I. Mannans produced in phosphate containing medium'. Together they form a unique fingerprint.

    Cite this