Structure of cell wall and exocellular mannans from the yeast Hansenula holstii. I. Mannans produced in phosphate containing medium

G San Blas, W L Cunningham

    Research output: Contribution to journalArticle

    Abstract

    Cell wall mannan and exocellular phosphomannan from Hansenula holstii NCYC 560 were analyzed to determine their structures. Both preparations were composed of a mixture of several polysaccharides (molecular weight = 1x106) differing in degree of phosphorylation and in structural arrangement. The cell wall mannan was a mixture of two branched polysaccharides containing 1,2 and 1,3 linkages, one of the fractions having ß linkages in addition to the a linkages usually found in these polymers. The exocellular phosphomannan was composed of three polymers, all of them with an a 1,6 linked backbone to which a 1,2 and a 1,3 linked side chains of various lengths were attached. The cell wall mannan contained phosphodiester linkages as reported for mannans from other species. However, the highly phosphorylated exocellular mannan was a novel kind of polysaccharide having its phosphates in the monoester form. It is concluded that the exocellular phosphomannan was not a product of autolysis of the cell wall material but rather an independently synthesized material

    Original languageEnglish
    Pages (from-to)233-246
    Number of pages14
    JournalBiochimica et Biophysica Acta - Gene Structure and Expression
    Volume354
    Issue number2
    Publication statusPublished - 1974

    Fingerprint

    Hansenula
    mannans
    cell walls
    phosphates
    yeasts
    polysaccharides
    polymers
    autolysis
    phosphorylation
    molecular weight

    Cite this

    @article{e3decc29ed2f498b8acd115ba171c95f,
    title = "Structure of cell wall and exocellular mannans from the yeast Hansenula holstii. I. Mannans produced in phosphate containing medium",
    abstract = "Cell wall mannan and exocellular phosphomannan from Hansenula holstii NCYC 560 were analyzed to determine their structures. Both preparations were composed of a mixture of several polysaccharides (molecular weight = 1x106) differing in degree of phosphorylation and in structural arrangement. The cell wall mannan was a mixture of two branched polysaccharides containing 1,2 and 1,3 linkages, one of the fractions having {\ss} linkages in addition to the a linkages usually found in these polymers. The exocellular phosphomannan was composed of three polymers, all of them with an a 1,6 linked backbone to which a 1,2 and a 1,3 linked side chains of various lengths were attached. The cell wall mannan contained phosphodiester linkages as reported for mannans from other species. However, the highly phosphorylated exocellular mannan was a novel kind of polysaccharide having its phosphates in the monoester form. It is concluded that the exocellular phosphomannan was not a product of autolysis of the cell wall material but rather an independently synthesized material",
    author = "{San Blas}, G and Cunningham, {W L}",
    year = "1974",
    language = "English",
    volume = "354",
    pages = "233--246",
    journal = "Biochimica et Biophysica Acta - Gene Regulatory Mechanisms",
    issn = "1874-9399",
    publisher = "Elsevier",
    number = "2",

    }

    TY - JOUR

    T1 - Structure of cell wall and exocellular mannans from the yeast Hansenula holstii. I. Mannans produced in phosphate containing medium

    AU - San Blas, G

    AU - Cunningham, W L

    PY - 1974

    Y1 - 1974

    N2 - Cell wall mannan and exocellular phosphomannan from Hansenula holstii NCYC 560 were analyzed to determine their structures. Both preparations were composed of a mixture of several polysaccharides (molecular weight = 1x106) differing in degree of phosphorylation and in structural arrangement. The cell wall mannan was a mixture of two branched polysaccharides containing 1,2 and 1,3 linkages, one of the fractions having ß linkages in addition to the a linkages usually found in these polymers. The exocellular phosphomannan was composed of three polymers, all of them with an a 1,6 linked backbone to which a 1,2 and a 1,3 linked side chains of various lengths were attached. The cell wall mannan contained phosphodiester linkages as reported for mannans from other species. However, the highly phosphorylated exocellular mannan was a novel kind of polysaccharide having its phosphates in the monoester form. It is concluded that the exocellular phosphomannan was not a product of autolysis of the cell wall material but rather an independently synthesized material

    AB - Cell wall mannan and exocellular phosphomannan from Hansenula holstii NCYC 560 were analyzed to determine their structures. Both preparations were composed of a mixture of several polysaccharides (molecular weight = 1x106) differing in degree of phosphorylation and in structural arrangement. The cell wall mannan was a mixture of two branched polysaccharides containing 1,2 and 1,3 linkages, one of the fractions having ß linkages in addition to the a linkages usually found in these polymers. The exocellular phosphomannan was composed of three polymers, all of them with an a 1,6 linked backbone to which a 1,2 and a 1,3 linked side chains of various lengths were attached. The cell wall mannan contained phosphodiester linkages as reported for mannans from other species. However, the highly phosphorylated exocellular mannan was a novel kind of polysaccharide having its phosphates in the monoester form. It is concluded that the exocellular phosphomannan was not a product of autolysis of the cell wall material but rather an independently synthesized material

    UR - http://www.scopus.com/inward/record.url?scp=0016236916&partnerID=8YFLogxK

    M3 - Article

    VL - 354

    SP - 233

    EP - 246

    JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

    JF - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

    SN - 1874-9399

    IS - 2

    ER -