Structure of cell wall and exocellular mannans from the yeast Hansenula holstii. II. Mannans produced in phosphate limited medium

G San Blas, W L Cunningham

    Research output: Contribution to journalArticle

    Abstract

    Cell wall mannan and exocellular phosphomannan from Hansenula holstii NCYC 560 grown under phosphate limited conditions were analyzed to determine their structures. Both preparations were homogeneous with respect to molecular weight (1x106), but heterogeneous toward ion exchange resins. Mannans studied were reduced in their phosphate content with O acetyl groups replacing most of the phosphates as constituents of these polymers. Both mannans had a branched structure similar to some extent to that described for Saccaromyces cerevisiae cell wall mannan, the main difference being the incorporation of O acetyl groups within the molecules. A role as ion exchanger in the outer regions of the cell wall is proposed for these mannans.

    Original languageEnglish
    Pages (from-to)247-253
    Number of pages7
    JournalBiochimica et Biophysica Acta - Gene Structure and Expression
    Volume354
    Issue number2
    Publication statusPublished - 1974

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