Structure-based Hamiltonian model for IsiA uncovers a highly robust pigment-protein complex

Hanan Schoffman, William M. Brown, Yossi Paltiel, Nir Keren, Erik M. Gauger

Research output: Contribution to journalArticle

Abstract

The iron stress-induced protein A (IsiA) is a source of interest and debate in biological research. The IsiA supercomplex, binding over 200 chlorophylls, assembles in multimeric rings around photosystem I (PSI). Recently, the IsiA-PSI structure from Synechocystis sp. PCC 6803 was resolved to 3.48 Å. Based on this structure, we created a model simulating a single excitation event in an IsiA monomer. This model enabled us to calculate the fluorescence and the localization of the excitation in the IsiA structure. To further examine this system, noise was introduced to the model in two forms-thermal and positional. Introducing noise highlights the functional differences in the system between cryogenic temperatures and biologically relevant temperatures. Our results show that the energetics of the IsiA pigment-protein complex are very robust at room temperature. Nevertheless, shifts in the position of specific chlorophylls lead to large changes in their optical and fluorescence properties. Based on these results, we discuss the implication of highly robust structures, with potential for serving different roles in a context-dependent manner, on our understanding of the function and evolution of photosynthetic processes.

Original languageEnglish
Article number20200399
JournalInterface
Volume17
Issue number169
Early online date26 Aug 2020
DOIs
Publication statusPublished - Aug 2020

Keywords

  • iron stress-induced protein A
  • photosynthetic complex
  • structure-based Hamiltonian model

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering

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