Structural alterations of erythrocyte membrane components induced by exhaustive exercise

Joanna Brzeszczywska, Anna Pieniazek, Lukasz Gwozdzinski, Krzysztof Gwozdzinski, Anna Jegier

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    16 Citations (Scopus)

    Abstract

    Physical exercise was used as a model of the physiological modulator of free radical production to examine the effects of exercise-induced oxidative modifications on the physico-biochemical properties of erythrocyte membrane. The aim of our work was to investigate conformational changes of erythrocyte membrane proteins, membrane fluidity, and membrane susceptibility to disintegration. Venous blood was taken before, immediately after, and 1 h after an exhaustive incremental cycling test (30 W·min-1 ramp), performed by 11 healthy untrained males on balanced diets (mean age, 22 ± 2 years; mean body mass index, 25 ± 4.5 kg·m-2). In response to this exercise, individual maximum heart rate was 195 ± 12 beats·min-1 and maximum wattage was 292 ± 27 W. Electron paramagnetic resonance spectroscopy was used to investigate alterations in membrane proteins and membrane dynamics, and to measure production of radical species. The reducing potential of plasma (RPP) was measured using the reduction of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the ferric-reducing ability of plasma. Exercise induced decreases in erythrocyte membrane fluidity in the polar region (p < 0.0001) and alterations in the conformational state of membrane proteins (p < 0.05). An increase in RPP was observed immediately after exercise (p < 0.001), with a further increase 1 h postexercise (p < 0.0001). Supporting measurements of lipid peroxidation showed an increase in thiobarbituric acid reactive substances immediately after exercise (p < 0.05) and at 1 h of recovery (p < 0.001); however, free radicals were not detected. Results indicate the existence of early postexercise mild oxidative stress after single-exercise performance, which induced structural changes in erythrocyte membrane components (protein aggregation) and in the membrane organization (lipids rigidization) that followed lipid peroxidation but did not lead to cellular hemolysis. © 2008 NRC.

    Original languageEnglish
    Pages (from-to)1223-1231
    Number of pages9
    JournalApplied Physiology, Nutrition and Metabolism
    Volume33
    Issue number6
    DOIs
    Publication statusPublished - Dec 2008

    Keywords

    • Cytoskeleton
    • EPR
    • Erythrocyte
    • Exercise
    • Free radicals
    • Oxidative stress
    • Spin labels

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  • Cite this

    Brzeszczywska, J., Pieniazek, A., Gwozdzinski, L., Gwozdzinski, K., & Jegier, A. (2008). Structural alterations of erythrocyte membrane components induced by exhaustive exercise. Applied Physiology, Nutrition and Metabolism, 33(6), 1223-1231. https://doi.org/10.1139/H08-125