Strawberry polyphenols are equally cytotoxic to tumourigenic and normal human breast and prostate cell lines

J. Weaver, T. Briscoe, M. Hou, C. Goodman, S. Kata, H.A. Ross, G.J. McDougall, D. Stewart, A. Riches

    Research output: Contribution to journalArticle

    Abstract

    The cytotoxic effects of strawberry polyphenols were investigated on normal cells and tumour cells derived from the same patient. A human prostate epithelial cell line (P21) and two tumour cell lines (P21 tumour cell line 1 and 2) derived from the same patient, and a normal human breast epithelial cell line (B42) and a tumour line derived from it (B42 clone 16) were used. A polyphenol-rich extract derived from strawberry or anthocyanin or tannin-rich sub-fractions were applied to the cell lines in doses varying from 50 to 1.5 µg/ml. The strawberry extract was cytotoxic with doses of ˜5 µg/ml causing a 50% reduction in cell survival in both the normal and the tumour lines. The extracts were also cytotoxic to peripheral blood human lymphocytes stimulated with phytohaemagglutinin but higher levels (>20 µg/ml for 50% reduction in cell survival) were required. After fractionation of the strawberry sample, the cytotoxicity was retained in the tannin-rich fraction and this fraction was considerably more toxic to all cells (normal or tumour cell lines or lymphocytes) than the anthocyanin-rich fraction. Established prostate (LNCaP and PC-3) and breast (MCF-7) tumour cell lines were more resistant to the strawberry extract with concentrations of 50 µg/ml required for 50% reduction in cell survival, which is similar to levels in previous studies on the antiproliferative effects of berry extracts. Although these concentrations are much greater than possible physiological levels, they are comparable to those reported in other studies. From these findings, we conclude that there is little evidence to assume that polyphenols from strawberry have a differential cytotoxic effect on tumour cells relative to comparable normal cells from the same tissue derived from the same patient.
    Copyright © 2013 Spandidos Publications Ltd.
    Original languageEnglish
    Pages (from-to)777-786
    Number of pages10
    JournalInternational Journal of Oncology
    Volume34
    Issue number3
    DOIs
    Publication statusPublished - Mar 2009

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    Fragaria
    Polyphenols
    Prostate
    Breast
    Tumor Cell Line
    Cell Line
    Cell Survival
    Anthocyanins
    Tannins
    Neoplasms
    Epithelial Cells
    Lymphocytes
    Poisons
    MCF-7 Cells
    Phytohemagglutinins
    Fruit
    Clone Cells

    Cite this

    Weaver, J. ; Briscoe, T. ; Hou, M. ; Goodman, C. ; Kata, S. ; Ross, H.A. ; McDougall, G.J. ; Stewart, D. ; Riches, A. / Strawberry polyphenols are equally cytotoxic to tumourigenic and normal human breast and prostate cell lines. In: International Journal of Oncology. 2009 ; Vol. 34, No. 3. pp. 777-786.
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    abstract = "The cytotoxic effects of strawberry polyphenols were investigated on normal cells and tumour cells derived from the same patient. A human prostate epithelial cell line (P21) and two tumour cell lines (P21 tumour cell line 1 and 2) derived from the same patient, and a normal human breast epithelial cell line (B42) and a tumour line derived from it (B42 clone 16) were used. A polyphenol-rich extract derived from strawberry or anthocyanin or tannin-rich sub-fractions were applied to the cell lines in doses varying from 50 to 1.5 µg/ml. The strawberry extract was cytotoxic with doses of ˜5 µg/ml causing a 50{\%} reduction in cell survival in both the normal and the tumour lines. The extracts were also cytotoxic to peripheral blood human lymphocytes stimulated with phytohaemagglutinin but higher levels (>20 µg/ml for 50{\%} reduction in cell survival) were required. After fractionation of the strawberry sample, the cytotoxicity was retained in the tannin-rich fraction and this fraction was considerably more toxic to all cells (normal or tumour cell lines or lymphocytes) than the anthocyanin-rich fraction. Established prostate (LNCaP and PC-3) and breast (MCF-7) tumour cell lines were more resistant to the strawberry extract with concentrations of 50 µg/ml required for 50{\%} reduction in cell survival, which is similar to levels in previous studies on the antiproliferative effects of berry extracts. Although these concentrations are much greater than possible physiological levels, they are comparable to those reported in other studies. From these findings, we conclude that there is little evidence to assume that polyphenols from strawberry have a differential cytotoxic effect on tumour cells relative to comparable normal cells from the same tissue derived from the same patient.Copyright {\circledC} 2013 Spandidos Publications Ltd.",
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    Weaver, J, Briscoe, T, Hou, M, Goodman, C, Kata, S, Ross, HA, McDougall, GJ, Stewart, D & Riches, A 2009, 'Strawberry polyphenols are equally cytotoxic to tumourigenic and normal human breast and prostate cell lines', International Journal of Oncology, vol. 34, no. 3, pp. 777-786. https://doi.org/10.3892/ijo_00000203

    Strawberry polyphenols are equally cytotoxic to tumourigenic and normal human breast and prostate cell lines. / Weaver, J.; Briscoe, T.; Hou, M.; Goodman, C.; Kata, S.; Ross, H.A.; McDougall, G.J.; Stewart, D.; Riches, A.

    In: International Journal of Oncology, Vol. 34, No. 3, 03.2009, p. 777-786.

    Research output: Contribution to journalArticle

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    AU - Weaver, J.

    AU - Briscoe, T.

    AU - Hou, M.

    AU - Goodman, C.

    AU - Kata, S.

    AU - Ross, H.A.

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    AU - Stewart, D.

    AU - Riches, A.

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    N2 - The cytotoxic effects of strawberry polyphenols were investigated on normal cells and tumour cells derived from the same patient. A human prostate epithelial cell line (P21) and two tumour cell lines (P21 tumour cell line 1 and 2) derived from the same patient, and a normal human breast epithelial cell line (B42) and a tumour line derived from it (B42 clone 16) were used. A polyphenol-rich extract derived from strawberry or anthocyanin or tannin-rich sub-fractions were applied to the cell lines in doses varying from 50 to 1.5 µg/ml. The strawberry extract was cytotoxic with doses of ˜5 µg/ml causing a 50% reduction in cell survival in both the normal and the tumour lines. The extracts were also cytotoxic to peripheral blood human lymphocytes stimulated with phytohaemagglutinin but higher levels (>20 µg/ml for 50% reduction in cell survival) were required. After fractionation of the strawberry sample, the cytotoxicity was retained in the tannin-rich fraction and this fraction was considerably more toxic to all cells (normal or tumour cell lines or lymphocytes) than the anthocyanin-rich fraction. Established prostate (LNCaP and PC-3) and breast (MCF-7) tumour cell lines were more resistant to the strawberry extract with concentrations of 50 µg/ml required for 50% reduction in cell survival, which is similar to levels in previous studies on the antiproliferative effects of berry extracts. Although these concentrations are much greater than possible physiological levels, they are comparable to those reported in other studies. From these findings, we conclude that there is little evidence to assume that polyphenols from strawberry have a differential cytotoxic effect on tumour cells relative to comparable normal cells from the same tissue derived from the same patient.Copyright © 2013 Spandidos Publications Ltd.

    AB - The cytotoxic effects of strawberry polyphenols were investigated on normal cells and tumour cells derived from the same patient. A human prostate epithelial cell line (P21) and two tumour cell lines (P21 tumour cell line 1 and 2) derived from the same patient, and a normal human breast epithelial cell line (B42) and a tumour line derived from it (B42 clone 16) were used. A polyphenol-rich extract derived from strawberry or anthocyanin or tannin-rich sub-fractions were applied to the cell lines in doses varying from 50 to 1.5 µg/ml. The strawberry extract was cytotoxic with doses of ˜5 µg/ml causing a 50% reduction in cell survival in both the normal and the tumour lines. The extracts were also cytotoxic to peripheral blood human lymphocytes stimulated with phytohaemagglutinin but higher levels (>20 µg/ml for 50% reduction in cell survival) were required. After fractionation of the strawberry sample, the cytotoxicity was retained in the tannin-rich fraction and this fraction was considerably more toxic to all cells (normal or tumour cell lines or lymphocytes) than the anthocyanin-rich fraction. Established prostate (LNCaP and PC-3) and breast (MCF-7) tumour cell lines were more resistant to the strawberry extract with concentrations of 50 µg/ml required for 50% reduction in cell survival, which is similar to levels in previous studies on the antiproliferative effects of berry extracts. Although these concentrations are much greater than possible physiological levels, they are comparable to those reported in other studies. From these findings, we conclude that there is little evidence to assume that polyphenols from strawberry have a differential cytotoxic effect on tumour cells relative to comparable normal cells from the same tissue derived from the same patient.Copyright © 2013 Spandidos Publications Ltd.

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