Stimulation of IL-8 release from epithelial cells by gas cooker PM10: a pilot study

C. A J Dick; M. Dennekamp; S. Howarth; J. W. Cherrie; A. Seaton; K. Donaldson; A. Stone

doi:10.1136/oem.58.3.208

Stimulation of IL-8 release from epithelial cells by gas cooker PM¹⁰: a pilot study

C. A J Dick^*, M. Dennekamp, S. Howarth, J. W. Cherrie, A. Seaton, K. Donaldson, A. Stone

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Objective - To measure the effect of matter collected by a method that has a 50% efficiency for particles with an aerodynamic diameter of 10 μm (PM₁₀), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO₂). Method - Multiple indoor PM₁₀ samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system. Results - Treatment of A549 cells with PM₁₀ generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO₂ did not alter the concentration of IL-8. Conclusion - PM₁₀ generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.

Original language	English
Pages (from-to)	208-210
Number of pages	3
Journal	Occupational and Environmental Medicine
Volume	58
Issue number	3
DOIs	https://doi.org/10.1136/oem.58.3.208
Publication status	Published - 12 Mar 2001

Keywords

Indoor air pollution
Interleukin-8
PM

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
General Environmental Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1136/oem.58.3.208

Cite this

@article{bcca1776276f4b5ea37bfc81bca0fcc8,

title = "Stimulation of IL-8 release from epithelial cells by gas cooker PM10: a pilot study",

abstract = "Objective - To measure the effect of matter collected by a method that has a 50\% efficiency for particles with an aerodynamic diameter of 10 μm (PM10), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO2). Method - Multiple indoor PM10 samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system. Results - Treatment of A549 cells with PM10 generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO2 did not alter the concentration of IL-8. Conclusion - PM10 generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.",

keywords = "Indoor air pollution, Interleukin-8, PM",

author = "Dick, \{C. A J\} and M. Dennekamp and S. Howarth and Cherrie, \{J. W.\} and A. Seaton and K. Donaldson and A. Stone",

year = "2001",

month = mar,

day = "12",

doi = "10.1136/oem.58.3.208",

language = "English",

volume = "58",

pages = "208--210",

journal = "Occupational and Environmental Medicine",

issn = "1351-0711",

publisher = "BMJ Publishing Group",

number = "3",

}

TY - JOUR

T1 - Stimulation of IL-8 release from epithelial cells by gas cooker PM10

T2 - a pilot study

AU - Dick, C. A J

AU - Dennekamp, M.

AU - Howarth, S.

AU - Cherrie, J. W.

AU - Seaton, A.

AU - Donaldson, K.

AU - Stone, A.

PY - 2001/3/12

Y1 - 2001/3/12

N2 - Objective - To measure the effect of matter collected by a method that has a 50% efficiency for particles with an aerodynamic diameter of 10 μm (PM10), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO2). Method - Multiple indoor PM10 samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system. Results - Treatment of A549 cells with PM10 generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO2 did not alter the concentration of IL-8. Conclusion - PM10 generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.

AB - Objective - To measure the effect of matter collected by a method that has a 50% efficiency for particles with an aerodynamic diameter of 10 μm (PM10), generated by gas and electric cooking, on A549 epithelial cells with and without nitrogen dioxide (NO2). Method - Multiple indoor PM10 samples were collected on Teflon filters during the use of gas or electric cookers. Interleukin-8 (IL-8) concentrations were measured with a sandwich enzyme linked immunosorbent assay (ELISA) system. Results - Treatment of A549 cells with PM10 generated from gas cooking resulted in increased concentrations of IL-8 compared with untreated cells; particles from the electric cooker had no effect. NO2 did not alter the concentration of IL-8. Conclusion - PM10 generated by gas cooking has the potential to cause proinflammatory effects in lung cells. This may have implications for susceptible people.

KW - Indoor air pollution

KW - Interleukin-8

KW - PM

UR - https://www.scopus.com/pages/publications/0035120688

U2 - 10.1136/oem.58.3.208

DO - 10.1136/oem.58.3.208

M3 - Article

C2 - 11171935

AN - SCOPUS:0035120688

SN - 1351-0711

VL - 58

SP - 208

EP - 210

JO - Occupational and Environmental Medicine

JF - Occupational and Environmental Medicine

IS - 3

ER -