SREBP-1c mediates the retinoid-dependent increase in fatty acid synthase promoter activity in HepG2

Karim Roder, Lei Zhang, Michael Schweizer

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    Treatment of HepG2 with all-trans retinoic acid (RA) induces expression of fatty acid synthase (FAS) mRNA and protein. Transfections show that the FAS promoter positively responds to retinoid X receptor (RXR) but not to RA receptor (RAR) agonists. Since RXR alone is capable of mediating the RA response of FAS, the existence of a classical RA-responsive element in the FAS promoter may be ruled out. Binding sites for NF-Y and SREBP-1 proved to be essential for the RA response. Exposure to all-trans RA increased mRNA and protein levels of SREBP-1, a transcriptional activator for FAS. Overexpression of a dominant-negative form of SREBP-1c diminished the RA-dependent increase in promoter activity. These data demonstrate that RXR ligands can stimulate the expression of a lipogenic gene solely by inducing transcription and cleavage of membrane-bound SREBP-1c. © 2007 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)2715-2720
    Number of pages6
    JournalFEBS Letters
    Volume581
    Issue number14
    DOIs
    Publication statusPublished - 12 Jun 2007

    Keywords

    • Fatty acid synthase
    • Retinoic acid
    • Sterol regulatory element-binding protein

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