A commercial enzyme preparation, of fungal origin, contained a mixture of ß-D-glucanases which were fractionated by ion-exchange chromatography to give a mixture of an endo-(1?4)- and an exo-(1?3)-ß-D-glucanase. These two enzymes were then separated by molecular-sieve chromatography on Sephadex G-150. The purified exo-(1?3)-ß-D-glucanase has a relatively high specificity for (1?3)-ß-D-glucosidic linkages, and has no action on lichenin. © 1976.
|Number of pages||6|
|Publication status||Published - Jul 1976|