Molecular-sieve chromatography of an extract from ungerminated rye indicated the presence of enzymes which hydrolysed cellobiose, laminaribiose and the ß-glucans cellodextrin, laminarin and barley ß-glucan. A purified endo-ß-1,3-glucanase was prepared from the extract by ammonium sulphate fractionation and molecular-sieve chromatography on Biogel P60. The substrate specificity and some properties of the enzyme are reported and the in vivo role of the enzyme is discussed. © 1973.
|Number of pages||3|
|Publication status||Published - Mar 1973|
- cereal β-glucans.
- Secale cereale