Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida

Jacinta M. Thornton; Dawn A. Austin; Brian Austin; Richard Powell

Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida

Jacinta M. Thornton, Dawn A. Austin, Brian Austin, Richard Powell

Research output: Contribution to journal › Article › peer-review

Abstract

The small subunit ribosomal RNA (SSU rRNA) encoding genes from reference strains of Aeromonas salmonicida subsp, smithia and Haemophilus piscium were amplified by polymerase chain reaction and cloned into Escherichia coil cells. Almost the entire SSU rRNA gene sequence (1505 nucleotides) from both organisms was determined. These DNA sequences were compared with those previously described from A. salmonicida subsp, salmonicida, subsp, achromogenes and subsp, masoucida. This genetic analysis revealed that A. salmonicida subsp. smithia and H. piscium showed 99.4 and 99.6% SSU rRNA gene sequence identity, respectively, with A. salmonicida subsp. salmonicida.

Original language	English
Pages (from-to)	155-158
Number of pages	4
Journal	Diseases of Aquatic Organisms
Volume	35
Issue number	2
Publication status	Published - 29 Jan 1999

Keywords

Aeromonas salmonicida
Haemophilus piscium
Ribosomal RNA genes

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@article{15987f61b47e4e238be8bb5947f11b13,

title = "Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida",

abstract = "The small subunit ribosomal RNA (SSU rRNA) encoding genes from reference strains of Aeromonas salmonicida subsp, smithia and Haemophilus piscium were amplified by polymerase chain reaction and cloned into Escherichia coil cells. Almost the entire SSU rRNA gene sequence (1505 nucleotides) from both organisms was determined. These DNA sequences were compared with those previously described from A. salmonicida subsp, salmonicida, subsp, achromogenes and subsp, masoucida. This genetic analysis revealed that A. salmonicida subsp. smithia and H. piscium showed 99.4 and 99.6% SSU rRNA gene sequence identity, respectively, with A. salmonicida subsp. salmonicida.",

keywords = "Aeromonas salmonicida, Haemophilus piscium, Ribosomal RNA genes",

author = "Thornton, {Jacinta M.} and Austin, {Dawn A.} and Brian Austin and Richard Powell",

year = "1999",

month = jan,

day = "29",

language = "English",

volume = "35",

pages = "155--158",

journal = "Diseases of Aquatic Organisms",

issn = "0177-5103",

publisher = "Inter-Research",

number = "2",

}

Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida. / Thornton, Jacinta M.; Austin, Dawn A.; Austin, Brian et al.
In: Diseases of Aquatic Organisms, Vol. 35, No. 2, 29.01.1999, p. 155-158.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida

AU - Thornton, Jacinta M.

AU - Austin, Dawn A.

AU - Austin, Brian

AU - Powell, Richard

PY - 1999/1/29

Y1 - 1999/1/29

N2 - The small subunit ribosomal RNA (SSU rRNA) encoding genes from reference strains of Aeromonas salmonicida subsp, smithia and Haemophilus piscium were amplified by polymerase chain reaction and cloned into Escherichia coil cells. Almost the entire SSU rRNA gene sequence (1505 nucleotides) from both organisms was determined. These DNA sequences were compared with those previously described from A. salmonicida subsp, salmonicida, subsp, achromogenes and subsp, masoucida. This genetic analysis revealed that A. salmonicida subsp. smithia and H. piscium showed 99.4 and 99.6% SSU rRNA gene sequence identity, respectively, with A. salmonicida subsp. salmonicida.

AB - The small subunit ribosomal RNA (SSU rRNA) encoding genes from reference strains of Aeromonas salmonicida subsp, smithia and Haemophilus piscium were amplified by polymerase chain reaction and cloned into Escherichia coil cells. Almost the entire SSU rRNA gene sequence (1505 nucleotides) from both organisms was determined. These DNA sequences were compared with those previously described from A. salmonicida subsp, salmonicida, subsp, achromogenes and subsp, masoucida. This genetic analysis revealed that A. salmonicida subsp. smithia and H. piscium showed 99.4 and 99.6% SSU rRNA gene sequence identity, respectively, with A. salmonicida subsp. salmonicida.

KW - Aeromonas salmonicida

KW - Haemophilus piscium

KW - Ribosomal RNA genes

M3 - Article

SN - 0177-5103

VL - 35

SP - 155

EP - 158

JO - Diseases of Aquatic Organisms

JF - Diseases of Aquatic Organisms

IS - 2

ER -

Small subunit rRNA gene sequences of Aeromonas salmonicida subsp. smithia and Haemophilus piscium reveal pronounced similarities with A. salmonicida subsp. salmonicida

Abstract

Keywords

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