Single Cell Isolation Using Optical Tweezers

Anusha Keloth, Owen Anderson, Donald Risbridger, Lynn Paterson

Research output: Contribution to journalArticle

Abstract

Optical tweezers offer a non-contact method for selecting single cells and translocating them from one microenvironment to another. We have characterized the optical tweezing of yeast S. cerevisiae and can manipulate single cells at 0.41 ± 0.06 mm/s using a 26.8 ± 0.1 mW from a 785 nm diode laser. We have fabricated and tested three cell isolation devices; a micropipette, a PDMS chip and a laser machined fused silica chip and we have isolated yeast, single bacteria and cyanobacteria cells. The most effective isolation was achieved in PDMS chips, where single yeast cells were grown and observed for 18 h without contamination. The duration of budding in S. cerevisiae was not affected by the laser parameters used, but the time from tweezing until the first budding event began increased with increasing laser energy (laser power × time). Yeast cells tweezed using 25.0 ± 0.1 mW for 1 min were viable after isolation. We have constructed a micro-consortium of yeast cells, and a co-culture of yeast and bacteria, using optical tweezers in combination with the PDMS network of channels and isolation chambers, which may impact on both industrial biotechnology and understanding pathogen dynamics.
Original languageEnglish
Article number434
JournalMicromachines
Volume9
Issue number9
DOIs
Publication statusPublished - 29 Aug 2018

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Optical Tweezers
Cell Separation
Yeasts
Lasers
Saccharomyces cerevisiae
Bacteria
Semiconductor Lasers
Cyanobacteria
Biotechnology
Coculture Techniques
Silicon Dioxide
Equipment and Supplies

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Keloth, Anusha ; Anderson, Owen ; Risbridger, Donald ; Paterson, Lynn. / Single Cell Isolation Using Optical Tweezers. In: Micromachines. 2018 ; Vol. 9, No. 9.
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Keloth, A, Anderson, O, Risbridger, D & Paterson, L 2018, 'Single Cell Isolation Using Optical Tweezers', Micromachines, vol. 9, no. 9, 434. https://doi.org/10.3390/mi9090434

Single Cell Isolation Using Optical Tweezers. / Keloth, Anusha; Anderson, Owen; Risbridger, Donald; Paterson, Lynn.

In: Micromachines, Vol. 9, No. 9, 434, 29.08.2018.

Research output: Contribution to journalArticle

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AB - Optical tweezers offer a non-contact method for selecting single cells and translocating them from one microenvironment to another. We have characterized the optical tweezing of yeast S. cerevisiae and can manipulate single cells at 0.41 ± 0.06 mm/s using a 26.8 ± 0.1 mW from a 785 nm diode laser. We have fabricated and tested three cell isolation devices; a micropipette, a PDMS chip and a laser machined fused silica chip and we have isolated yeast, single bacteria and cyanobacteria cells. The most effective isolation was achieved in PDMS chips, where single yeast cells were grown and observed for 18 h without contamination. The duration of budding in S. cerevisiae was not affected by the laser parameters used, but the time from tweezing until the first budding event began increased with increasing laser energy (laser power × time). Yeast cells tweezed using 25.0 ± 0.1 mW for 1 min were viable after isolation. We have constructed a micro-consortium of yeast cells, and a co-culture of yeast and bacteria, using optical tweezers in combination with the PDMS network of channels and isolation chambers, which may impact on both industrial biotechnology and understanding pathogen dynamics.

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