Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Alexandra C. Adams; András Kufcsák; Katjana Ehrlich; Kevin Dhaliwal; Sohan Seth

doi:10.1109/tbme.2023.3244664

Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Alexandra C. Adams, András Kufcsák, Katjana Ehrlich, Kevin Dhaliwal, Sohan Seth

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

69 Downloads (Pure)

Abstract

Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.

Original language	English
Pages (from-to)	2395-2403
Number of pages	9
Journal	IEEE Transactions on Biomedical Engineering
Volume	70
Issue number	8
Early online date	13 Mar 2023
DOIs	https://doi.org/10.1109/tbme.2023.3244664
Publication status	Published - Aug 2023

Keywords

B-splines
Fluorescence lifetime
non-linear least squares
time-resolved fluor- escence spectroscopy

ASJC Scopus subject areas

Biomedical Engineering

Access to Document

10.1109/tbme.2023.3244664

Download pdf
© 2023 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Accepted author manuscript, 22.3 MB

Cite this

@article{db6a2e11843d4e269c1271cb9ee7f714,

title = "Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum",

abstract = "Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.",

keywords = "B-splines, Fluorescence lifetime, non-linear least squares, time-resolved fluor- escence spectroscopy",

author = "Adams, {Alexandra C.} and Andr{\'a}s Kufcs{\'a}k and Katjana Ehrlich and Kevin Dhaliwal and Sohan Seth",

year = "2023",

month = aug,

doi = "10.1109/tbme.2023.3244664",

language = "English",

volume = "70",

pages = "2395--2403",

journal = "IEEE Transactions on Biomedical Engineering",

issn = "0018-9294",

publisher = "IEEE",

number = "8",

}

TY - JOUR

T1 - Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

AU - Adams, Alexandra C.

AU - Kufcsák, András

AU - Ehrlich, Katjana

AU - Dhaliwal, Kevin

AU - Seth, Sohan

PY - 2023/8

Y1 - 2023/8

N2 - Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.

AB - Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.

KW - B-splines

KW - Fluorescence lifetime

KW - non-linear least squares

KW - time-resolved fluor- escence spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85151566784&partnerID=8YFLogxK

U2 - 10.1109/tbme.2023.3244664

DO - 10.1109/tbme.2023.3244664

M3 - Article

SN - 0018-9294

VL - 70

SP - 2395

EP - 2403

JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

IS - 8

ER -

Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this