Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Alexandra C. Adams, András Kufcsák, Katjana Ehrlich, Kevin Dhaliwal, Sohan Seth

Research output: Contribution to journalArticlepeer-review

12 Downloads (Pure)


Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.
Original languageEnglish
Pages (from-to)2395-2403
Number of pages9
JournalIEEE Transactions on Biomedical Engineering
Issue number8
Early online date13 Mar 2023
Publication statusPublished - Aug 2023


  • B-splines
  • Fluorescence lifetime
  • non-linear least squares
  • time-resolved fluor- escence spectroscopy

ASJC Scopus subject areas

  • Biomedical Engineering


Dive into the research topics of 'Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum'. Together they form a unique fingerprint.

Cite this