Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

A. E. Carruthers; Tanya K. Lake; Lynn Paterson; J. W. Allen; Wilson Sibbett; Kishan Dholakia

doi:10.1109/CLEOE.2003.1313780

Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

A. E. Carruthers, Tanya K. Lake, Lynn Paterson, J. W. Allen, Wilson Sibbett, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.

Original language	English
Title of host publication	2003 Conference on Lasers and Electro-Optics Europe, 2003. CLEO/Europe
Publisher	IEEE
Pages	712
Number of pages	1
ISBN (Print)	0780377346, 9780780377349
DOIs	https://doi.org/10.1109/CLEOE.2003.1313780
Publication status	Published - 2003
Event	2003 Conference on Lasers and Electro-Optics Europe - Munich, Germany Duration: 22 Jun 2003 → 27 Jun 2003

Conference

Conference	2003 Conference on Lasers and Electro-Optics Europe
Country/Territory	Germany
City	Munich
Period	22/06/03 → 27/06/03

ASJC Scopus subject areas

Electrical and Electronic Engineering
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials

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10.1109/CLEOE.2003.1313780

Cite this

@inproceedings{da7417d4ef6f44c3a1c8bde2c999929a,

title = "Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes",

abstract = "In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.",

author = "Carruthers, \{A. E.\} and Lake, \{Tanya K.\} and Lynn Paterson and Allen, \{J. W.\} and Wilson Sibbett and Kishan Dholakia",

year = "2003",

doi = "10.1109/CLEOE.2003.1313780",

language = "English",

isbn = "0780377346",

pages = "712",

booktitle = "2003 Conference on Lasers and Electro-Optics Europe, 2003. CLEO/Europe",

publisher = "IEEE",

address = "United States",

note = "2003 Conference on Lasers and Electro-Optics Europe ; Conference date: 22-06-2003 Through 27-06-2003",

}

Carruthers, AE, Lake, TK, Paterson, L, Allen, JW, Sibbett, W & Dholakia, K 2003, Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes. in 2003 Conference on Lasers and Electro-Optics Europe, 2003. CLEO/Europe. IEEE, pp. 712, 2003 Conference on Lasers and Electro-Optics Europe, Munich, Germany, 22/06/03. https://doi.org/10.1109/CLEOE.2003.1313780

TY - GEN

T1 - Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

AU - Carruthers, A. E.

AU - Lake, Tanya K.

AU - Paterson, Lynn

AU - Allen, J. W.

AU - Sibbett, Wilson

AU - Dholakia, Kishan

PY - 2003

Y1 - 2003

N2 - In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.

AB - In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.

UR - https://www.scopus.com/pages/publications/84891852330

U2 - 10.1109/CLEOE.2003.1313780

DO - 10.1109/CLEOE.2003.1313780

M3 - Conference contribution

AN - SCOPUS:84891852330

SN - 0780377346

SN - 9780780377349

SP - 712

BT - 2003 Conference on Lasers and Electro-Optics Europe, 2003. CLEO/Europe

PB - IEEE

T2 - 2003 Conference on Lasers and Electro-Optics Europe

Y2 - 22 June 2003 through 27 June 2003

ER -

Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

Abstract

Conference

ASJC Scopus subject areas

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