Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes

A. E. Carruthers, Tanya K. Lake, Lynn Paterson, J. W. Allen, Wilson Sibbett, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.

Original languageEnglish
Title of host publication2003 Conference on Lasers and Electro-Optics Europe, 2003. CLEO/Europe
PublisherIEEE
Pages712
Number of pages1
ISBN (Print)0780377346, 9780780377349
DOIs
Publication statusPublished - 2003
Event2003 Conference on Lasers and Electro-Optics Europe - Munich, Germany
Duration: 22 Jun 200327 Jun 2003

Conference

Conference2003 Conference on Lasers and Electro-Optics Europe
CountryGermany
CityMunich
Period22/06/0327/06/03

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials

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  • Cite this

    Carruthers, A. E., Lake, T. K., Paterson, L., Allen, J. W., Sibbett, W., & Dholakia, K. (2003). Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes. In 2003 Conference on Lasers and Electro-Optics Europe, 2003. CLEO/Europe (pp. 712). IEEE. https://doi.org/10.1109/CLEOE.2003.1313780