SERS as a tool for in vitro toxicology

Kate M. Fisher, Jennifer A. McLeish, Lauren E. Jamieson, Jing Jiang, James R. Hopgood, Stephen McLaughlin, Ken Donaldson, Colin J. Campbell

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Measuring markers of stress such as pH and redox potential are important when studying toxicology in in vitro models because they are markers of oxidative stress, apoptosis and viability. While surface enhanced Raman spectroscopy is ideally suited to the measurement of redox potential and pH in live cells, the time-intensive nature and perceived difficulty in signal analysis and interpretation can be a barrier to its broad uptake by the biological community. In this paper we detail the development of signal processing and analysis algorithms that allow SERS spectra to be automatically processed so that the output of the processing is a pH or redox potential value. By automating signal processing we were able to carry out a comparative evaluation of the toxicology of silver and zinc oxide nanoparticles and correlate our findings with qPCR analysis. The combination of these two analytical techniques sheds light on the differences in toxicology between these two materials from the perspective of oxidative stress.

Original languageEnglish
Pages (from-to)501-520
Number of pages20
JournalFaraday Discussions
Volume187
Early online date28 Jan 2016
DOIs
Publication statusPublished - 1 Jun 2016

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Fingerprint Dive into the research topics of 'SERS as a tool for in vitro toxicology'. Together they form a unique fingerprint.

  • Cite this

    Fisher, K. M., McLeish, J. A., Jamieson, L. E., Jiang, J., Hopgood, J. R., McLaughlin, S., Donaldson, K., & Campbell, C. J. (2016). SERS as a tool for in vitro toxicology. Faraday Discussions, 187, 501-520. https://doi.org/10.1039/c5fd00216h