Separation by dielectrophoresis of dormant and nondormant bacterial cells of Mycobacterium smegmatis

Ke Zhu, Arseny S. Kaprelyants, Elena G. Salina, Gerard H. Markx

Research output: Contribution to journalArticle

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Abstract

The dielectrophoretic behavior of active, dead, and dormant Mycobacterium smegmatis bacterial cells was studied. It was found that the 72-h-old dormant cells had a much higher effective particle conductivity (812±10 µS cm-1), almost double that of active cells (560±20 µS cm-1), while that of dead (autoclaved) M. smegmatis cells was the highest (950±15 µS cm-1) overall. It was also found that at 80 kHz, 900 µS cm-1 dead cells were attracted at the edges of interdigitated castellated electrodes by positive dielectrophoresis, but dormant cells were not. Similarly, at 120 kHz, 2 µS cm-1 active cells were attracted and dormant cells were not. Using these findings a dielectrophoresis-based microfluidic separation system was developed in which dead and active cells were collected from a given cell suspension, while dormant cells were eluted. © 2010 American Institute of Physics.

Original languageEnglish
Article number007095BMF
Pages (from-to)9-11
Number of pages3
JournalBiomicrofluidics
Volume4
Issue number2
DOIs
Publication statusPublished - Jun 2010

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