Selective plane illumination optical endomicroscopy with polymer imaging fibers

Pablo Roldán-Varona, Calum Ross, Luis Rodríguez-Cobo, José Miguel López-Higuera, Erin Gaughan, Kevin Dhaliwal, Michael G Tanner, Robert R. Thomson, Helen Parker

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
38 Downloads (Pure)

Abstract

Imaging fibers are used to perform real-time fluorescence endomicroscopy, in vivo, in situ, with the goal of increasing diagnostic information for a plethora of organ systems and diseases. Widefield fiber endomicroscopy systems are simple, cost-effective, and come with fast image acquisition times. However, alternative approaches such as scanning systems produce higher contrast images with intrinsic optical sectioning, improving the visibility of histological features, albeit at the expense of simplicity, cost, and acquisition rate. We developed a selective plane illumination microscopy endoscopic fiber platform, consisting of an ultrafast laser fabricated end-cap, integrated with a polymer coherent fiber bundle, and an epifluorescence microscope. Polymer fibers are known to fluoresce when pumped with blue light, enhancing the background and noise in images. Our end-cap design circumvents this challenge. We demonstrate a reduction of out-of-focus features, along with improved contrast of in-focus features, in images of a tissue phantom. Moreover, we demonstrate the utility of our platform for endomicroscopy using a whole, ex vivo human lung model.

Original languageEnglish
Article number016103
JournalAPL Photonics
Volume8
Issue number1
DOIs
Publication statusPublished - 13 Jan 2023

Keywords

  • Atomic and Molecular Physics, and Optics
  • Computer Networks and Communications

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Computer Networks and Communications

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