Scale-up of enzymatic solid-to-solid peptide synthesis and enzyme recovery

Markus Erbeldinger, Peter J. Halling, Xiongwei Ni

Research output: Contribution to journalArticle

Abstract

The feasibility of scale-up for applications of enzymatic solid-to-solid synthesis of dipeptides was investigated by using Thermoase catalyzed synthesis of Z-Asp-Phe-OMe as a model reaction. The reaction system involves typically only 10% (w/w) enzyme suspension added to dry solid substrates. Enzymatic solid-to-solid biocatalysis combines the main advantages of aqueous media, high catalytic activity, low water media, and high yields. A flow sheet for the whole process is described, as well as the use of a jacketed reactor with an anchor stirrer for various steps of the process, including the neutralization reaction to adjust the acid-base condition of the reaction mixture. A solution for enzyme recycling, an important issue for scale-up applications, is also suggested.

Original languageEnglish
Pages (from-to)500-508
Number of pages9
JournalAIChE Journal
Volume47
Issue number2
DOIs
Publication statusPublished - 2001

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Recovery
Peptides
Enzymes
Dipeptides
Anchors
Recycling
Catalyst activity
Suspensions
Acids
Water
Substrates

Cite this

Erbeldinger, Markus ; Halling, Peter J. ; Ni, Xiongwei. / Scale-up of enzymatic solid-to-solid peptide synthesis and enzyme recovery. In: AIChE Journal. 2001 ; Vol. 47, No. 2. pp. 500-508.
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Scale-up of enzymatic solid-to-solid peptide synthesis and enzyme recovery. / Erbeldinger, Markus; Halling, Peter J.; Ni, Xiongwei.

In: AIChE Journal, Vol. 47, No. 2, 2001, p. 500-508.

Research output: Contribution to journalArticle

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