Role of Sp1 and Sp3 in the transcriptional regulation of the rat fatty acid synthase gene

Siegmund S. Wolf, Karim Roder, Michael Schweizer

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Inspection of the 5' region of the sequence of the rat fatty acid synthase (FAS) gene revealed a high GC content between -900 and +500, implying several binding sites for members of the Sp1 family of transcription factors. Using SL2 and H4IIE cells in conjunction with FAS promoter/luciferase constructs either successively deleted or containing defined deletions we characterized six GC boxes - GC-I to GC-VI - located between -557 and -83 and discovered a seventh, GC-VII, in the first intron. In vitro DNAse I-footprinting, electrophoretic mobility shift assays, and the yeast one-hybrid system indicated that Sp1 as well as Sp3 interacts with GC-I to GC-VII. Each of the GC boxes conferred Sp1-dependent transcription on the FAS-Mini promoter and in the case of GC-I, Sp1, and Sp3 exert an additive effect on FAS promoter activity. © 2001 Academic Press.

    Original languageEnglish
    Pages (from-to)259-266
    Number of pages8
    JournalArchives of Biochemistry and Biophysics
    Volume385
    Issue number2
    DOIs
    Publication statusPublished - 15 Jan 2001

    Keywords

    • EMSA
    • Fatty acid synthase
    • Gene regulation Sp1
    • H4IIE cells
    • Schneider cell line
    • Sp3
    • SREBP
    • Yeast one-hybrid system

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