Abstract
Inspection of the 5' region of the sequence of the rat fatty acid synthase (FAS) gene revealed a high GC content between -900 and +500, implying several binding sites for members of the Sp1 family of transcription factors. Using SL2 and H4IIE cells in conjunction with FAS promoter/luciferase constructs either successively deleted or containing defined deletions we characterized six GC boxes - GC-I to GC-VI - located between -557 and -83 and discovered a seventh, GC-VII, in the first intron. In vitro DNAse I-footprinting, electrophoretic mobility shift assays, and the yeast one-hybrid system indicated that Sp1 as well as Sp3 interacts with GC-I to GC-VII. Each of the GC boxes conferred Sp1-dependent transcription on the FAS-Mini promoter and in the case of GC-I, Sp1, and Sp3 exert an additive effect on FAS promoter activity. © 2001 Academic Press.
Original language | English |
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Pages (from-to) | 259-266 |
Number of pages | 8 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 385 |
Issue number | 2 |
DOIs | |
Publication status | Published - 15 Jan 2001 |
Keywords
- EMSA
- Fatty acid synthase
- Gene regulation Sp1
- H4IIE cells
- Schneider cell line
- Sp3
- SREBP
- Yeast one-hybrid system