Rescue of fish exposed to a lethal dose of pathogen, by signals from sublethally exposed survivors

Carmel Mothersill, Dawn Austin, Cristian Fernandez-Palomo, Colin Seymour, Niall Auchinachie, Brian Austin

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    6 Citations (Scopus)

    Abstract

    Rainbow trout (Oncorhynchus mykiss, Walbaum) were challenged intraperitoneally with a sublethal dose of Vibrio anguillarum VIB1 and allowed to recover. Then, after 7 days, naive fish, (designated as 'bystander' fish) which had never been exposed to the pathogen, were introduced to the same tank. These swam with the adapted (recovered) fish for 7 days before both groups and a control (never exposed directly to the pathogen or to recovered fish) group were exposed to a lethal dose of VIB1. Mortality records were 100% in the control group within 3 days, 47% in the adapted group and 60% in the unchallenged bystander group, which swam with the adapted group. In both the latter groups, the time to death of the non-surviving fish was attenuated. This inter-animal communication of signals has previously been documented for animals exposed to ionizing radiation. Assays of tissues from control, challenged and ` bystander fish exposed to the pathogen showed that a signal as yet unidentified but similar to that seen in bystanders to irradiated fish was being produced. This signal caused a sharp and transient increase in intracellular calcium and a decrease in clonogenicity in a well-characterized reporter assay.

    Original languageEnglish
    Article number058
    Number of pages5
    JournalFEMS Microbiology Letters
    Volume362
    Issue number5
    Early online date12 Dec 2014
    DOIs
    Publication statusPublished - Mar 2015

    Keywords

    • Vibrio anguillarum
    • rainbow trout
    • bystander effect
    • sublethal exposure
    • disease
    • VIBRIO-ANGUILLARUM
    • BYSTANDER SIGNALS
    • IN-VIVO
    • RADIATION
    • STRESS
    • IDENTIFICATION
    • IRRADIATION
    • PROTECTION
    • ISSUES

    Cite this

    Mothersill, C., Austin, D., Fernandez-Palomo, C., Seymour, C., Auchinachie, N., & Austin, B. (2015). Rescue of fish exposed to a lethal dose of pathogen, by signals from sublethally exposed survivors. FEMS Microbiology Letters, 362(5), [058]. https://doi.org/10.1093/femsle/fnu058