Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo

Barbara Rothen-Rutishauser; David M Brown; Matthew Piallier-Boyles; Ian A Kinloch; Alan H Windle; Peter Gehr; Vicki Stone

doi:10.3109/17435390.2010.489161

Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo

Barbara Rothen-Rutishauser, David M Brown, Matthew Piallier-Boyles, Ian A Kinloch, Alan H Windle, Peter Gehr, Vicki Stone

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) production is important in the toxicity of pathogenic particles such as fibres. We examined the oxidative potential of straight (50 microm and 10 microm) and tangled carbon nanotubes in a cell free assay, in vitro and in vivo using different dispersants. The cell free oxidative potential of tangled nanotubes was higher than for the straight fibres. In cultured macrophages tangled tubes exhibited significantly more ROS at 30 min, while straight tubes increased ROS at 4 h. ROS was significantly higher in bronchoalveolar lavage cells of animals instilled with tangled and 10 mum straight fibres, whereas the number of neutrophils increased only in animals treated with the long tubes. Addition of dispersants in the suspension media lead to enhanced ROS detection by entangled tubes in the cell-free system. Tangled fibres generated more ROS in a cell-free system and in cultured cells, while straight fibres generated a slower but more prolonged effect in animals.

Original language	English
Pages (from-to)	331-42
Number of pages	12
Journal	Nanotoxicology
Volume	4
Issue number	3
DOIs	https://doi.org/10.3109/17435390.2010.489161
Publication status	Published - 2010

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Rothen-Rutishauser, B., Brown, D. M., Piallier-Boyles, M., Kinloch, I. A., Windle, A. H., Gehr, P., & Stone, V. (2010). Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo. Nanotoxicology, 4(3), 331-42. https://doi.org/10.3109/17435390.2010.489161

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title = "Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo",

abstract = "Reactive oxygen species (ROS) production is important in the toxicity of pathogenic particles such as fibres. We examined the oxidative potential of straight (50 microm and 10 microm) and tangled carbon nanotubes in a cell free assay, in vitro and in vivo using different dispersants. The cell free oxidative potential of tangled nanotubes was higher than for the straight fibres. In cultured macrophages tangled tubes exhibited significantly more ROS at 30 min, while straight tubes increased ROS at 4 h. ROS was significantly higher in bronchoalveolar lavage cells of animals instilled with tangled and 10 mum straight fibres, whereas the number of neutrophils increased only in animals treated with the long tubes. Addition of dispersants in the suspension media lead to enhanced ROS detection by entangled tubes in the cell-free system. Tangled fibres generated more ROS in a cell-free system and in cultured cells, while straight fibres generated a slower but more prolonged effect in animals.",

author = "Barbara Rothen-Rutishauser and Brown, {David M} and Matthew Piallier-Boyles and Kinloch, {Ian A} and Windle, {Alan H} and Peter Gehr and Vicki Stone",

year = "2010",

doi = "10.3109/17435390.2010.489161",

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Rothen-Rutishauser, B, Brown, DM, Piallier-Boyles, M, Kinloch, IA, Windle, AH, Gehr, P & Stone, V 2010, 'Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo', Nanotoxicology, vol. 4, no. 3, pp. 331-42. https://doi.org/10.3109/17435390.2010.489161

Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo. / Rothen-Rutishauser, Barbara; Brown, David M; Piallier-Boyles, Matthew et al.
In: Nanotoxicology, Vol. 4, No. 3, 2010, p. 331-42.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo

AU - Rothen-Rutishauser, Barbara

AU - Brown, David M

AU - Piallier-Boyles, Matthew

AU - Kinloch, Ian A

AU - Windle, Alan H

AU - Gehr, Peter

AU - Stone, Vicki

PY - 2010

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N2 - Reactive oxygen species (ROS) production is important in the toxicity of pathogenic particles such as fibres. We examined the oxidative potential of straight (50 microm and 10 microm) and tangled carbon nanotubes in a cell free assay, in vitro and in vivo using different dispersants. The cell free oxidative potential of tangled nanotubes was higher than for the straight fibres. In cultured macrophages tangled tubes exhibited significantly more ROS at 30 min, while straight tubes increased ROS at 4 h. ROS was significantly higher in bronchoalveolar lavage cells of animals instilled with tangled and 10 mum straight fibres, whereas the number of neutrophils increased only in animals treated with the long tubes. Addition of dispersants in the suspension media lead to enhanced ROS detection by entangled tubes in the cell-free system. Tangled fibres generated more ROS in a cell-free system and in cultured cells, while straight fibres generated a slower but more prolonged effect in animals.

AB - Reactive oxygen species (ROS) production is important in the toxicity of pathogenic particles such as fibres. We examined the oxidative potential of straight (50 microm and 10 microm) and tangled carbon nanotubes in a cell free assay, in vitro and in vivo using different dispersants. The cell free oxidative potential of tangled nanotubes was higher than for the straight fibres. In cultured macrophages tangled tubes exhibited significantly more ROS at 30 min, while straight tubes increased ROS at 4 h. ROS was significantly higher in bronchoalveolar lavage cells of animals instilled with tangled and 10 mum straight fibres, whereas the number of neutrophils increased only in animals treated with the long tubes. Addition of dispersants in the suspension media lead to enhanced ROS detection by entangled tubes in the cell-free system. Tangled fibres generated more ROS in a cell-free system and in cultured cells, while straight fibres generated a slower but more prolonged effect in animals.

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DO - 10.3109/17435390.2010.489161

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SN - 1743-5404

VL - 4

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EP - 342

JO - Nanotoxicology

JF - Nanotoxicology

IS - 3

ER -

Relating the physicochemical characteristics and dispersion of multiwalled carbon nanotubes in different suspension media to their oxidative reactivity in vitro and inflammation in vivo

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