Regulation of polyglutamic acid synthesis by glutamate in Bacillus licheniformis and Bacillus subtilis

M. Kambourova, M. Tangney, F. G. Priest

    Research output: Contribution to journalArticle

    Abstract

    The synthesis of polyglutamic acid (PGA) was repressed by exogenous glutamate in strains of Bacillus licheniformis but not in strains of Bacillus subtilis, indicating a clear difference in the regulation of synthesis of capsular slime in these two species. Although extracellular ?-glutamyltranspeptidase (GGT) activity was always present in PGA-producing cultures of B. licheniformis under various growth conditions, there was no correlation between the quantity of PGA and enzyme activity. Moreover, the synthesis of PGA in the absence of detectable GGT activity in B. subtilis S317 indicated that this enzyme was not involved in PGA biosynthesis in this bacterium. Glutamate repression of PGA biosynthesis may offer a simple means of preventing unwanted slime production in industrial fermentations using B. licheniformis.

    Original languageEnglish
    Pages (from-to)1004-1007
    Number of pages4
    JournalApplied and Environmental Microbiology
    Volume67
    Issue number2
    DOIs
    Publication statusPublished - 2001

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    polyglutamic acid
    Bacillus licheniformis
    glutamates
    Bacillus subtilis
    synthesis
    biosynthesis
    fermentation
    enzyme activity
    bacteria
    enzymes

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    abstract = "The synthesis of polyglutamic acid (PGA) was repressed by exogenous glutamate in strains of Bacillus licheniformis but not in strains of Bacillus subtilis, indicating a clear difference in the regulation of synthesis of capsular slime in these two species. Although extracellular ?-glutamyltranspeptidase (GGT) activity was always present in PGA-producing cultures of B. licheniformis under various growth conditions, there was no correlation between the quantity of PGA and enzyme activity. Moreover, the synthesis of PGA in the absence of detectable GGT activity in B. subtilis S317 indicated that this enzyme was not involved in PGA biosynthesis in this bacterium. Glutamate repression of PGA biosynthesis may offer a simple means of preventing unwanted slime production in industrial fermentations using B. licheniformis.",
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    Regulation of polyglutamic acid synthesis by glutamate in Bacillus licheniformis and Bacillus subtilis. / Kambourova, M.; Tangney, M.; Priest, F. G.

    In: Applied and Environmental Microbiology, Vol. 67, No. 2, 2001, p. 1004-1007.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Regulation of polyglutamic acid synthesis by glutamate in Bacillus licheniformis and Bacillus subtilis

    AU - Kambourova, M.

    AU - Tangney, M.

    AU - Priest, F. G.

    PY - 2001

    Y1 - 2001

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    AB - The synthesis of polyglutamic acid (PGA) was repressed by exogenous glutamate in strains of Bacillus licheniformis but not in strains of Bacillus subtilis, indicating a clear difference in the regulation of synthesis of capsular slime in these two species. Although extracellular ?-glutamyltranspeptidase (GGT) activity was always present in PGA-producing cultures of B. licheniformis under various growth conditions, there was no correlation between the quantity of PGA and enzyme activity. Moreover, the synthesis of PGA in the absence of detectable GGT activity in B. subtilis S317 indicated that this enzyme was not involved in PGA biosynthesis in this bacterium. Glutamate repression of PGA biosynthesis may offer a simple means of preventing unwanted slime production in industrial fermentations using B. licheniformis.

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