Regulation of mitochondrial function and biogenesis in cucumber (Cucumis sativus L.) Cotyledons during early seedling growth

Steven A. Hill, C. P L Grof, James H. Bryce, Christopher J. Leaver

    Research output: Contribution to journalArticle

    Abstract

    The aim of this work was to characterize the respiratory metabolism of the greening cotyledons of cucumber (Cucumis sativus L.) during early seedling growth and to investigate how this is integrated with changes in mitochondrial biogenesis and function. In light-grown cotyledons, lipid mobilization extended from germination to 6 days postimbibition, reaching a maximum at 3 to 4 days postimbibition. The rate of dark oxygen uptake reached a maximum at 2 days postimbibition in dark-grown and 3 days postimbibition in light-grown cotyledons. Development of photosynthetic capacity occurred from 4 to 7 days postimbibition. In dark-grown cotyledons, lipid mobilization extended beyond 7 days postimbibition, and there was no greening or acquisition of photosynthetic competence. Measurements of mitochondrial function indicated that the respiratory capacity of the tissue changed such that during lipid mobilization there was a much greater capacity for the operation of the nondecarboxylating portion of the citric acid cycle (succinate to oxaloacetate), whereas during the development of photosynthetic function the activity of the remainder of the cycle (oxaloacetate to succinate) was induced. Comparison of the maximum capacities for mitochondrial substrate oxidations in vitro with the rates of in vivo substrate oxidations, predicted from the rate of lipid breakdown, indicated that mitochondria in this tissue operate at or below state 4 rates, suggesting limitation by both availability of ADP and substrate.

    Original languageEnglish
    Pages (from-to)60-66
    Number of pages7
    JournalPlant Physiology
    Volume99
    Issue number1
    Publication statusPublished - 1992

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    Cucumis sativus
    cucumbers
    seedling growth
    cotyledons
    succinic acid
    lipids
    oxidation
    tricarboxylic acid cycle
    lung function
    mitochondria
    germination
    uptake mechanisms
    oxygen
    metabolism
    biogenesis
    tissues

    Cite this

    Hill, Steven A. ; Grof, C. P L ; Bryce, James H. ; Leaver, Christopher J. / Regulation of mitochondrial function and biogenesis in cucumber (Cucumis sativus L.) Cotyledons during early seedling growth. In: Plant Physiology. 1992 ; Vol. 99, No. 1. pp. 60-66.
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    abstract = "The aim of this work was to characterize the respiratory metabolism of the greening cotyledons of cucumber (Cucumis sativus L.) during early seedling growth and to investigate how this is integrated with changes in mitochondrial biogenesis and function. In light-grown cotyledons, lipid mobilization extended from germination to 6 days postimbibition, reaching a maximum at 3 to 4 days postimbibition. The rate of dark oxygen uptake reached a maximum at 2 days postimbibition in dark-grown and 3 days postimbibition in light-grown cotyledons. Development of photosynthetic capacity occurred from 4 to 7 days postimbibition. In dark-grown cotyledons, lipid mobilization extended beyond 7 days postimbibition, and there was no greening or acquisition of photosynthetic competence. Measurements of mitochondrial function indicated that the respiratory capacity of the tissue changed such that during lipid mobilization there was a much greater capacity for the operation of the nondecarboxylating portion of the citric acid cycle (succinate to oxaloacetate), whereas during the development of photosynthetic function the activity of the remainder of the cycle (oxaloacetate to succinate) was induced. Comparison of the maximum capacities for mitochondrial substrate oxidations in vitro with the rates of in vivo substrate oxidations, predicted from the rate of lipid breakdown, indicated that mitochondria in this tissue operate at or below state 4 rates, suggesting limitation by both availability of ADP and substrate.",
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    Regulation of mitochondrial function and biogenesis in cucumber (Cucumis sativus L.) Cotyledons during early seedling growth. / Hill, Steven A.; Grof, C. P L; Bryce, James H.; Leaver, Christopher J.

    In: Plant Physiology, Vol. 99, No. 1, 1992, p. 60-66.

    Research output: Contribution to journalArticle

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    AU - Leaver, Christopher J.

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