Redox-active lipid-incorporating proteins as a novel immobilisation technique

Claus Jacob, Alexander Y Safronov, Sonia Wilson, H Allen O Hill, Tim F Booth, Stephen K Chapman

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    4 Citations (Scopus)

    Abstract

    A novel chiral redox-active ferrocene compound (FcV1) with amphiphilic properties has been synthesised and used successfully to form protein + lipid 'clusters' with ferritin and gold-labelled BSA. These structures could be observed by cryogenic transmission electron microscopy and had diameters between 40 and 300 nm. It was shown that the novel lipid formed reasonably stable films on an edge plane graphite electrode surface and also mediated the electron transfer between flavocytochrome b(2) and this electrode. Consequently, the enzyme was incorporated into an FcV1 film and its response towards L(+)lactate in solution investigated. It could be shown that the electrode indeed was sensitive to L(+)lactate. (C) 1997 Elsevier Science S.A.

    Original languageEnglish
    Pages (from-to)119-125
    Number of pages7
    JournalJournal of Electroanalytical Chemistry
    Volume430
    Issue number1-2
    DOIs
    Publication statusPublished - 30 Jun 1997

    Keywords

    • LOCALIZATION
    • flavocytochrome bz
    • SURFACTANT
    • FLAVOCYTOCHROME-B2
    • cryo-TEM
    • ORGANOMETALLIC AMPHIPHILES
    • GLUCOSE-OXIDASE
    • lipid incorporating proteins
    • FERROCENE
    • MEDIATION
    • mediation
    • VESICLES
    • ELECTRON-MICROSCOPY
    • BILAYER

    Cite this

    Jacob, C., Safronov, A. Y., Wilson, S., Hill, H. A. O., Booth, T. F., & Chapman, S. K. (1997). Redox-active lipid-incorporating proteins as a novel immobilisation technique. Journal of Electroanalytical Chemistry, 430(1-2), 119-125. https://doi.org/10.1016/S0022-0728(97)00102-2