Recovery of a new biogroup of Yersinia ruckeri from diseased rainbow trout (Oncorhynchus mykiss, Walbaum)

D. A. Austin, P. A W Robertson, B. Austin

    Research output: Contribution to journalArticle

    Abstract

    Cultures of a new biogroup of Yersinia ruckeri, the causal agent of enteric redmouth (ERM), were recovered in England from diseased rainbow-trout (Oncorhynchus mykiss, Walbaum), which had been previously vaccinated with a commercial ERM vaccine. The bacterial isolates were confirmed as Y. ruckeri by the results of sequencing the 16S rRNA, but differed from the characteristics of the taxon by positivity for the Voges Proskauer reaction and a general lack of motility, and could not be equated with any of the existing serovars. Cultures were pathogenic in laboratory-based infectivity experiments with 100% mortalities occurring in juvenile rainbow trout (average weight = 10 g) within 4-days of intraperitoneal or intramuscular injection with 105 cells/fish. Protection against disease was achieved using a formalin-inactivated whole vaccine prepared against a representative isolate.

    Original languageEnglish
    Pages (from-to)127-131
    Number of pages5
    JournalSystematic and Applied Microbiology
    Volume26
    Issue number1
    DOIs
    Publication statusPublished - Mar 2003

    Fingerprint

    Yersinia ruckeri
    Oncorhynchus mykiss
    vaccines
    intraperitoneal injection
    intramuscular injection
    formalin
    England
    serotypes
    pathogenicity
    ribosomal RNA
    fish
    cells

    Keywords

    • Fish disease
    • New biogroup
    • Rainbow trout
    • Yersinia ruckeri

    Cite this

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    abstract = "Cultures of a new biogroup of Yersinia ruckeri, the causal agent of enteric redmouth (ERM), were recovered in England from diseased rainbow-trout (Oncorhynchus mykiss, Walbaum), which had been previously vaccinated with a commercial ERM vaccine. The bacterial isolates were confirmed as Y. ruckeri by the results of sequencing the 16S rRNA, but differed from the characteristics of the taxon by positivity for the Voges Proskauer reaction and a general lack of motility, and could not be equated with any of the existing serovars. Cultures were pathogenic in laboratory-based infectivity experiments with 100{\%} mortalities occurring in juvenile rainbow trout (average weight = 10 g) within 4-days of intraperitoneal or intramuscular injection with 105 cells/fish. Protection against disease was achieved using a formalin-inactivated whole vaccine prepared against a representative isolate.",
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    author = "Austin, {D. A.} and Robertson, {P. A W} and B. Austin",
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    Recovery of a new biogroup of Yersinia ruckeri from diseased rainbow trout (Oncorhynchus mykiss, Walbaum). / Austin, D. A.; Robertson, P. A W; Austin, B.

    In: Systematic and Applied Microbiology, Vol. 26, No. 1, 03.2003, p. 127-131.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Austin, D. A.

    AU - Robertson, P. A W

    AU - Austin, B.

    PY - 2003/3

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    AB - Cultures of a new biogroup of Yersinia ruckeri, the causal agent of enteric redmouth (ERM), were recovered in England from diseased rainbow-trout (Oncorhynchus mykiss, Walbaum), which had been previously vaccinated with a commercial ERM vaccine. The bacterial isolates were confirmed as Y. ruckeri by the results of sequencing the 16S rRNA, but differed from the characteristics of the taxon by positivity for the Voges Proskauer reaction and a general lack of motility, and could not be equated with any of the existing serovars. Cultures were pathogenic in laboratory-based infectivity experiments with 100% mortalities occurring in juvenile rainbow trout (average weight = 10 g) within 4-days of intraperitoneal or intramuscular injection with 105 cells/fish. Protection against disease was achieved using a formalin-inactivated whole vaccine prepared against a representative isolate.

    KW - Fish disease

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    KW - Rainbow trout

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