Abstract
We demonstrate a microfluidic system in which magnetic particles are used as mobile solid supports for performing multi-step bioreactions in continuous flow. A mouse IgG sandwich immunoassay has been successfully demonstrated in which a biotinylated primary antibody was bound to the surface of streptavidin coated parti-cles and then labelled by a fluorescently tagged secondary antibody, all within 60 s. This system greatly reduces procedural times commonly associated with bioanalysis and the progression of an assay can be monitored on a particle by particle basis.
| Original language | English |
|---|---|
| Pages | 1114-1116 |
| Number of pages | 3 |
| Publication status | Published - 2008 |
| Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008 - San Diego, United States Duration: 12 Oct 2008 → 16 Oct 2008 |
Conference
| Conference | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2008 |
|---|---|
| Abbreviated title | MicroTAS 2008 |
| Country/Territory | United States |
| City | San Diego |
| Period | 12/10/08 → 16/10/08 |
Keywords
- Assays
- Continuous flow
- Magnetic deflection
- Microparticles
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering