Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry

Kevin McLean; Javier Palarea-Albaladejo; Carol G. Currie; Lisa H. J. Imrie; Erin D. T. Manson; Douglas Fraser-Pitt; Frank Wright; Colin James Alexander; Kevin G. J. Pollock; Lesley Allison; Mary Hanson; David G. E. Smith

doi:10.1016/j.talanta.2018.01.055

Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry

Kevin McLean, Javier Palarea-Albaladejo, Carol G. Currie, Lisa H. J. Imrie, Erin D. T. Manson, Douglas Fraser-Pitt, Frank Wright, Colin James Alexander, Kevin G. J. Pollock, Lesley Allison, Mary Hanson, David G. E. Smith

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios.

Original language	English
Pages (from-to)	164-170
Number of pages	7
Journal	Talanta
Volume	182
Early online date	20 Feb 2018
DOIs	https://doi.org/10.1016/j.talanta.2018.01.055
Publication status	Published - 15 May 2018

Keywords

Escherichia coli
MALDI-MS
Mass spectrometry
Shigatoxigenic E. coli
Spectral processing
STEC

ASJC Scopus subject areas

Chemistry(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.talanta.2018.01.055

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McLean, K., Palarea-Albaladejo, J., Currie, C. G., Imrie, L. H. J., Manson, E. D. T., Fraser-Pitt, D., Wright, F., Alexander, C. J., Pollock, K. G. J., Allison, L., Hanson, M., & Smith, D. G. E. (2018). Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry. Talanta, 182, 164-170. https://doi.org/10.1016/j.talanta.2018.01.055

McLean, Kevin ; Palarea-Albaladejo, Javier ; Currie, Carol G. ; Imrie, Lisa H. J. ; Manson, Erin D. T. ; Fraser-Pitt, Douglas ; Wright, Frank ; Alexander, Colin James ; Pollock, Kevin G. J. ; Allison, Lesley ; Hanson, Mary ; Smith, David G. E. / Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry. In: Talanta. 2018 ; Vol. 182. pp. 164-170.

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title = "Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry",

abstract = "Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios.",

keywords = "Escherichia coli, MALDI-MS, Mass spectrometry, Shigatoxigenic E. coli, Spectral processing, STEC",

author = "Kevin McLean and Javier Palarea-Albaladejo and Currie, {Carol G.} and Imrie, {Lisa H. J.} and Manson, {Erin D. T.} and Douglas Fraser-Pitt and Frank Wright and Alexander, {Colin James} and Pollock, {Kevin G. J.} and Lesley Allison and Mary Hanson and Smith, {David G. E.}",

year = "2018",

month = may,

day = "15",

doi = "10.1016/j.talanta.2018.01.055",

language = "English",

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McLean, K, Palarea-Albaladejo, J, Currie, CG, Imrie, LHJ, Manson, EDT, Fraser-Pitt, D, Wright, F, Alexander, CJ, Pollock, KGJ, Allison, L, Hanson, M & Smith, DGE 2018, 'Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry', Talanta, vol. 182, pp. 164-170. https://doi.org/10.1016/j.talanta.2018.01.055

Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry. / McLean, Kevin; Palarea-Albaladejo, Javier; Currie, Carol G.; Imrie, Lisa H. J.; Manson, Erin D. T.; Fraser-Pitt, Douglas; Wright, Frank; Alexander, Colin James; Pollock, Kevin G. J.; Allison, Lesley; Hanson, Mary; Smith, David G. E.

In: Talanta, Vol. 182, 15.05.2018, p. 164-170.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry

AU - McLean, Kevin

AU - Palarea-Albaladejo, Javier

AU - Currie, Carol G.

AU - Imrie, Lisa H. J.

AU - Manson, Erin D. T.

AU - Fraser-Pitt, Douglas

AU - Wright, Frank

AU - Alexander, Colin James

AU - Pollock, Kevin G. J.

AU - Allison, Lesley

AU - Hanson, Mary

AU - Smith, David G. E.

PY - 2018/5/15

Y1 - 2018/5/15

N2 - Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios.

AB - Whole cell MALDI is regularly used for the identification of bacteria to species level in clinical Microbiology laboratories. However, there remains a need to rapidly characterize and differentiate isolates below the species level to support outbreak management. We describe the implementation of a modified preparative approach for MALDI-MS combined with a custom analytical computational pipeline as a rapid procedure for subtyping Shigatoxigenic E. coli (STEC) and accurately identifying strain-specifying biomarkers. The technique was able to differentiate E. coli O157:H7 from other STEC. Within O157 serotype O157:H7 isolates were readily distinguishable from Sorbitol Fermenting O157 isolates. Overall, nine homogeneous groups of isolates were distinguished, each exhibiting distinct profiles of defining mass spectra features. This offers a robust analytical tool useable in reference/diagnostic public health scenarios.

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KW - MALDI-MS

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JO - Talanta

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SN - 0039-9140

ER -

Rapid and robust analytical protocol for E. coli STEC bacteria subspecies differentiation using whole cell MALDI mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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