Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 2162-2170 |
| Number of pages | 9 |
| Journal | Small |
| Volume | 9 |
| Issue number | 12 |
| Early online date | 11 Feb 2013 |
| DOIs | |
| Publication status | Published - 24 Jun 2013 |
Keywords
- nanothermometry
- optical trapping
- quantum dots
- microspheres
- single cells
- RESONANCE RAMAN-SPECTROSCOPY
- LASER TWEEZERS
- WAVELENGTH DEPENDENCE
- MANIPULATION
- TEMPERATURE
- APOPTOSIS
- NECROSIS
- FORCES
- DEATH
Cite this
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Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells. / Haro-Gonzalez, Patricia; Ramsay, William T.; Martinez Maestro, Laura; del Rosal, Blanca; Santacruz-Gomez, Karla; del Carmen Iglesias-de la Cruz, Maria; Sanz-Rodriguez, Francisco; Chooi, Jing Yuang; Rodriguez Sevilla, Paloma; Bettinelli, Marco; Choudhury, Debaditya; Kar, Ajoy Kumar; Garcia Sole, Jose; Jaque, Daniel; Paterson, Lynn.
In: Small, Vol. 9, No. 12, 24.06.2013, p. 2162-2170.Research output: Contribution to journal › Article
TY - JOUR
T1 - Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells
AU - Haro-Gonzalez, Patricia
AU - Ramsay, William T.
AU - Martinez Maestro, Laura
AU - del Rosal, Blanca
AU - Santacruz-Gomez, Karla
AU - del Carmen Iglesias-de la Cruz, Maria
AU - Sanz-Rodriguez, Francisco
AU - Chooi, Jing Yuang
AU - Rodriguez Sevilla, Paloma
AU - Bettinelli, Marco
AU - Choudhury, Debaditya
AU - Kar, Ajoy Kumar
AU - Garcia Sole, Jose
AU - Jaque, Daniel
AU - Paterson, Lynn
N1 - Accepted, available early online 11 February 2013
PY - 2013/6/24
Y1 - 2013/6/24
N2 - Laser-induced thermal effects in optically trapped micro-spheres and single cells have been investigated by Quantum Dot Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Quantum Dot Luminescence Thermometry has demonstrated that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43ºC), thus, avoiding cell damage.
AB - Laser-induced thermal effects in optically trapped micro-spheres and single cells have been investigated by Quantum Dot Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Quantum Dot Luminescence Thermometry has demonstrated that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43ºC), thus, avoiding cell damage.
KW - nanothermometry
KW - optical trapping
KW - quantum dots
KW - microspheres
KW - single cells
KW - RESONANCE RAMAN-SPECTROSCOPY
KW - LASER TWEEZERS
KW - WAVELENGTH DEPENDENCE
KW - MANIPULATION
KW - TEMPERATURE
KW - APOPTOSIS
KW - NECROSIS
KW - FORCES
KW - DEATH
U2 - 10.1002/smll.201201740
DO - 10.1002/smll.201201740
M3 - Article
VL - 9
SP - 2162
EP - 2170
JO - Small
JF - Small
SN - 1613-6829
IS - 12
ER -