Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells

Patricia Haro-Gonzalez, William T. Ramsay, Laura Martinez Maestro, Blanca del Rosal, Karla Santacruz-Gomez, Maria del Carmen Iglesias-de la Cruz, Francisco Sanz-Rodriguez, Jing Yuang Chooi, Paloma Rodriguez Sevilla, Marco Bettinelli, Debaditya Choudhury, Ajoy Kumar Kar, Jose Garcia Sole, Daniel Jaque, Lynn Paterson

Research output: Contribution to journalArticle

Abstract

Laser-induced thermal effects in optically trapped micro-spheres and single cells have been investigated by Quantum Dot Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Quantum Dot Luminescence Thermometry has demonstrated that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43ºC), thus, avoiding cell damage.
Original languageEnglish
Pages (from-to)2162-2170
Number of pages9
JournalSmall
Volume9
Issue number12
Early online date11 Feb 2013
DOIs
Publication statusPublished - 24 Jun 2013

Keywords

  • nanothermometry
  • optical trapping
  • quantum dots
  • microspheres
  • single cells
  • RESONANCE RAMAN-SPECTROSCOPY
  • LASER TWEEZERS
  • WAVELENGTH DEPENDENCE
  • MANIPULATION
  • TEMPERATURE
  • APOPTOSIS
  • NECROSIS
  • FORCES
  • DEATH

Cite this

Haro-Gonzalez, P., Ramsay, W. T., Martinez Maestro, L., del Rosal, B., Santacruz-Gomez, K., del Carmen Iglesias-de la Cruz, M., ... Paterson, L. (2013). Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells. Small, 9(12), 2162-2170. https://doi.org/10.1002/smll.201201740
Haro-Gonzalez, Patricia ; Ramsay, William T. ; Martinez Maestro, Laura ; del Rosal, Blanca ; Santacruz-Gomez, Karla ; del Carmen Iglesias-de la Cruz, Maria ; Sanz-Rodriguez, Francisco ; Chooi, Jing Yuang ; Rodriguez Sevilla, Paloma ; Bettinelli, Marco ; Choudhury, Debaditya ; Kar, Ajoy Kumar ; Garcia Sole, Jose ; Jaque, Daniel ; Paterson, Lynn. / Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells. In: Small. 2013 ; Vol. 9, No. 12. pp. 2162-2170.
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title = "Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells",
abstract = "Laser-induced thermal effects in optically trapped micro-spheres and single cells have been investigated by Quantum Dot Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Quantum Dot Luminescence Thermometry has demonstrated that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43ºC), thus, avoiding cell damage.",
keywords = "nanothermometry, optical trapping, quantum dots, microspheres, single cells, RESONANCE RAMAN-SPECTROSCOPY, LASER TWEEZERS, WAVELENGTH DEPENDENCE, MANIPULATION, TEMPERATURE, APOPTOSIS, NECROSIS, FORCES, DEATH",
author = "Patricia Haro-Gonzalez and Ramsay, {William T.} and {Martinez Maestro}, Laura and {del Rosal}, Blanca and Karla Santacruz-Gomez and {del Carmen Iglesias-de la Cruz}, Maria and Francisco Sanz-Rodriguez and Chooi, {Jing Yuang} and {Rodriguez Sevilla}, Paloma and Marco Bettinelli and Debaditya Choudhury and Kar, {Ajoy Kumar} and {Garcia Sole}, Jose and Daniel Jaque and Lynn Paterson",
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Haro-Gonzalez, P, Ramsay, WT, Martinez Maestro, L, del Rosal, B, Santacruz-Gomez, K, del Carmen Iglesias-de la Cruz, M, Sanz-Rodriguez, F, Chooi, JY, Rodriguez Sevilla, P, Bettinelli, M, Choudhury, D, Kar, AK, Garcia Sole, J, Jaque, D & Paterson, L 2013, 'Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells', Small, vol. 9, no. 12, pp. 2162-2170. https://doi.org/10.1002/smll.201201740

Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells. / Haro-Gonzalez, Patricia; Ramsay, William T.; Martinez Maestro, Laura; del Rosal, Blanca; Santacruz-Gomez, Karla; del Carmen Iglesias-de la Cruz, Maria; Sanz-Rodriguez, Francisco; Chooi, Jing Yuang; Rodriguez Sevilla, Paloma; Bettinelli, Marco; Choudhury, Debaditya; Kar, Ajoy Kumar; Garcia Sole, Jose; Jaque, Daniel; Paterson, Lynn.

In: Small, Vol. 9, No. 12, 24.06.2013, p. 2162-2170.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells

AU - Haro-Gonzalez, Patricia

AU - Ramsay, William T.

AU - Martinez Maestro, Laura

AU - del Rosal, Blanca

AU - Santacruz-Gomez, Karla

AU - del Carmen Iglesias-de la Cruz, Maria

AU - Sanz-Rodriguez, Francisco

AU - Chooi, Jing Yuang

AU - Rodriguez Sevilla, Paloma

AU - Bettinelli, Marco

AU - Choudhury, Debaditya

AU - Kar, Ajoy Kumar

AU - Garcia Sole, Jose

AU - Jaque, Daniel

AU - Paterson, Lynn

N1 - Accepted, available early online 11 February 2013

PY - 2013/6/24

Y1 - 2013/6/24

N2 - Laser-induced thermal effects in optically trapped micro-spheres and single cells have been investigated by Quantum Dot Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Quantum Dot Luminescence Thermometry has demonstrated that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43ºC), thus, avoiding cell damage.

AB - Laser-induced thermal effects in optically trapped micro-spheres and single cells have been investigated by Quantum Dot Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Quantum Dot Luminescence Thermometry has demonstrated that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43ºC), thus, avoiding cell damage.

KW - nanothermometry

KW - optical trapping

KW - quantum dots

KW - microspheres

KW - single cells

KW - RESONANCE RAMAN-SPECTROSCOPY

KW - LASER TWEEZERS

KW - WAVELENGTH DEPENDENCE

KW - MANIPULATION

KW - TEMPERATURE

KW - APOPTOSIS

KW - NECROSIS

KW - FORCES

KW - DEATH

U2 - 10.1002/smll.201201740

DO - 10.1002/smll.201201740

M3 - Article

VL - 9

SP - 2162

EP - 2170

JO - Small

JF - Small

SN - 1613-6829

IS - 12

ER -

Haro-Gonzalez P, Ramsay WT, Martinez Maestro L, del Rosal B, Santacruz-Gomez K, del Carmen Iglesias-de la Cruz M et al. Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells. Small. 2013 Jun 24;9(12):2162-2170. https://doi.org/10.1002/smll.201201740