Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells

Patricia Haro-Gonzalez, William T. Ramsay, Laura Martinez Maestro, Blanca del Rosal, Karla Santacruz-Gomez, Maria del Carmen Iglesias-de la Cruz, Francisco Sanz-Rodriguez, Jing Yuang Chooi, Paloma Rodriguez Sevilla, Marco Bettinelli, Debaditya Choudhury, Ajoy Kumar Kar, Jose Garcia Sole, Daniel Jaque, Lynn Paterson

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53 Citations (Scopus)

Abstract

Laser-induced thermal effects in optically trapped micro-spheres and single cells have been investigated by Quantum Dot Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Quantum Dot Luminescence Thermometry has demonstrated that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43ºC), thus, avoiding cell damage.
Original languageEnglish
Pages (from-to)2162-2170
Number of pages9
JournalSmall
Volume9
Issue number12
Early online date11 Feb 2013
DOIs
Publication statusPublished - 24 Jun 2013

Keywords

  • nanothermometry
  • optical trapping
  • quantum dots
  • microspheres
  • single cells
  • RESONANCE RAMAN-SPECTROSCOPY
  • LASER TWEEZERS
  • WAVELENGTH DEPENDENCE
  • MANIPULATION
  • TEMPERATURE
  • APOPTOSIS
  • NECROSIS
  • FORCES
  • DEATH

Cite this

Haro-Gonzalez, P., Ramsay, W. T., Martinez Maestro, L., del Rosal, B., Santacruz-Gomez, K., del Carmen Iglesias-de la Cruz, M., Sanz-Rodriguez, F., Chooi, J. Y., Rodriguez Sevilla, P., Bettinelli, M., Choudhury, D., Kar, A. K., Garcia Sole, J., Jaque, D., & Paterson, L. (2013). Quantum dot based thermal spectroscopy and imaging of optically trapped micro-spheres and single cells. Small, 9(12), 2162-2170. https://doi.org/10.1002/smll.201201740