Quantitative trait loci associated with different polar metabolites in perennial ryegrass - providing scope for breeding towards increasing certain polar metabolites

Alexandre Foito, Christine Anne Hackett, Derek Stewart, Janaki Velmurugan, Dan Milbourne, Stephen L. Byrne, Susanne Barth

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Abstract

Background: Recent advances in the mapping of biochemical traits have been reported in Lolium perenne. Although the mapped traits, including individual sugars and fatty acids, contribute greatly towards ruminant productivity, organic acids and amino acids have been largely understudied despite their influence on the ruminal microbiome. Results: In this study, we used a targeted gas-chromatography mass spectrometry (GC-MS) approach to profile the levels of 25 polar metabolites from different classes (sugars, amino acids, phenolic acids, organic acids and other nitrogen-containing compounds) present in a L. perenne F2 population consisting of 325 individuals. A quantitative trait (QTL) mapping approach was applied and successfully identified QTLs regulating seven of those polar metabolites (L-serine, L-leucine, glucose, fructose, myo-inositol, citric acid and 2, 3-hydroxypropanoic acid).Two QTL mapping approaches were carried out using SNP markers on about half of the population only and an imputation approach using SNP and DArT markers on the entire population. The imputation approach confirmed the four QTLs found in the SNP-only analysis and identified a further seven QTLs. Conclusions: These results highlight the potential of utilising molecular assisted breeding in perennial ryegrass to modulate a range of biochemical quality traits with downstream effects in livestock productivity and ruminal digestion.

Original languageEnglish
Article number84
JournalBMC Genetics
Volume18
DOIs
Publication statusPublished - 10 Oct 2017

Keywords

  • 3-hydroxypropanoic acid
  • Aminoacids
  • Citric acid and 2
  • Lolium perenne
  • Myo-inositol
  • Perennial ryegrass
  • Polar metabolites
  • QTL
  • Soluble carbohydrates

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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