Abstract
In this work we present data showing deformability-based cell separation in a deterministic lateral displacement (DLD) device. We use cells of defined stiffness (glutaraldehyde cross-linked erythrocytes) to test the performance of the device across a range of cell stiffness and applied shear rates. Optical stretching is used as an independent method of quantifying the stiffness of the cells, thus allowing better understanding of the DLD system for deformability based cell separation. Displacement is shown to correlate with cell stiffness as measured across a range of flow rates. Data showing how the isolation of leukocytes from whole blood varies with shear rate is also presented.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 536-538 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 2012 |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2012 - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012 |
Conference
| Conference | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2012 |
|---|---|
| Abbreviated title | MicroTAS 2012 |
| Country/Territory | Japan |
| City | Okinawa |
| Period | 28/10/12 → 1/11/12 |
Keywords
- Blood
- Cell deformability
- Deterministic lateral displacement
- Optical stretching
- Shear rate
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering