Quantitative analysis of deformability-based cell separation using deterministic lateral displacement and optical stretching

David Holmes, Graeme Whyte, Andrew Ekpenyong, Jochen Guck, Tom Duke

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this work we present data showing deformability-based cell separation in a deterministic lateral displacement (DLD) device. We use cells of defined stiffness (glutaraldehyde cross-linked erythrocytes) to test the performance of the device across a range of cell stiffness and applied shear rates. Optical stretching is used as an independent method of quantifying the stiffness of the cells, thus allowing better understanding of the DLD system for deformability based cell separation. Displacement is shown to correlate with cell stiffness as measured across a range of flow rates. Data showing how the isolation of leukocytes from whole blood varies with shear rate is also presented.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages536-538
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2012
Abbreviated titleMicroTAS 2012
CountryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Blood
  • Cell deformability
  • Deterministic lateral displacement
  • Optical stretching
  • Shear rate

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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