Purification of malted-barley endo-β-D-glucanases by ion-exchange chromatography: some properties of an endo-barley-β-D-glucanase

Two endo-ß-D-glucanases which act, respectively, on (1?3)-ß-D-glucans and barley ß-D-glucan have been isolated from malted barley, and purified by ion-exchange chromatography. The latter enzyme is highly specific for barley ß-D-glucan, and has no action on either (1?3)- or (1?4)-ß-D-glucans. It will also act on dyed barley-ß-D-glucan. Certain group-specific reagents inhibit the endo-barley-ß-D-glucanase and the endo-(1?3)-ß-D-glucanase to similar extents. © 1976.