Purification of malted-barley endo-β-D-glucanases by ion-exchange chromatography

some properties of an endo-barley-β-D-glucanase

David J Manners, Glynn Wilson

    Research output: Contribution to journalArticle

    Abstract

    Two endo-ß-D-glucanases which act, respectively, on (1?3)-ß-D-glucans and barley ß-D-glucan have been isolated from malted barley, and purified by ion-exchange chromatography. The latter enzyme is highly specific for barley ß-D-glucan, and has no action on either (1?3)- or (1?4)-ß-D-glucans. It will also act on dyed barley-ß-D-glucan. Certain group-specific reagents inhibit the endo-barley-ß-D-glucanase and the endo-(1?3)-ß-D-glucanase to similar extents. © 1976.

    Original languageEnglish
    Pages (from-to)255-264
    Number of pages10
    JournalCarbohydrate Research
    Volume48
    Issue number2
    Publication statusPublished - Jun 1976

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    Chromatography
    Purification
    Ion exchange
    polyglucosan
    Enzymes

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    title = "Purification of malted-barley endo-β-D-glucanases by ion-exchange chromatography: some properties of an endo-barley-β-D-glucanase",
    abstract = "Two endo-{\ss}-D-glucanases which act, respectively, on (1?3)-{\ss}-D-glucans and barley {\ss}-D-glucan have been isolated from malted barley, and purified by ion-exchange chromatography. The latter enzyme is highly specific for barley {\ss}-D-glucan, and has no action on either (1?3)- or (1?4)-{\ss}-D-glucans. It will also act on dyed barley-{\ss}-D-glucan. Certain group-specific reagents inhibit the endo-barley-{\ss}-D-glucanase and the endo-(1?3)-{\ss}-D-glucanase to similar extents. {\circledC} 1976.",
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    Purification of malted-barley endo-β-D-glucanases by ion-exchange chromatography : some properties of an endo-barley-β-D-glucanase. / Manners, David J; Wilson, Glynn.

    In: Carbohydrate Research, Vol. 48, No. 2, 06.1976, p. 255-264.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Purification of malted-barley endo-β-D-glucanases by ion-exchange chromatography

    T2 - some properties of an endo-barley-β-D-glucanase

    AU - Manners, David J

    AU - Wilson, Glynn

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