Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity

Eleanor Watson, Aileen Sherry, Neil F. Inglis, Alex Lainson, Dushyanth Jyothi, Raja Yaga, Erin Manson, Lisa Imrie, Paul Everest, David George Emslie Smith

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith-Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.

    Original languageEnglish
    Pages (from-to)184-194
    Number of pages11
    JournalEuPA Open Proteomics
    Volume4
    DOIs
    Publication statusPublished - Sep 2014

    Keywords

    • Campylobacter jejuni
    • Genomics
    • Outer membrane
    • Proteomics

    ASJC Scopus subject areas

    • Biochemistry

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