Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity

Eleanor Watson; Aileen Sherry; Neil F. Inglis; Alex Lainson; Dushyanth Jyothi; Raja Yaga; Erin Manson; Lisa Imrie; Paul Everest; David George Emslie Smith

doi:10.1016/j.euprot.2014.06.003

Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity

Eleanor Watson^*, Aileen Sherry, Neil F. Inglis, Alex Lainson, Dushyanth Jyothi, Raja Yaga, Erin Manson, Lisa Imrie, Paul Everest, David George Emslie Smith

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith-Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.

Original language	English
Pages (from-to)	184-194
Number of pages	11
Journal	EuPA Open Proteomics
Volume	4
DOIs	https://doi.org/10.1016/j.euprot.2014.06.003
Publication status	Published - Sept 2014

Keywords

Campylobacter jejuni
Genomics
Outer membrane
Proteomics

ASJC Scopus subject areas

Biochemistry

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10.1016/j.euprot.2014.06.003

Cite this

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title = "Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity",

abstract = "Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith-Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.",

keywords = "Campylobacter jejuni, Genomics, Outer membrane, Proteomics",

author = "Eleanor Watson and Aileen Sherry and Inglis, {Neil F.} and Alex Lainson and Dushyanth Jyothi and Raja Yaga and Erin Manson and Lisa Imrie and Paul Everest and Smith, {David George Emslie}",

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language = "English",

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T1 - Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity

AU - Watson, Eleanor

AU - Sherry, Aileen

AU - Inglis, Neil F.

AU - Lainson, Alex

AU - Jyothi, Dushyanth

AU - Yaga, Raja

AU - Manson, Erin

AU - Imrie, Lisa

AU - Everest, Paul

AU - Smith, David George Emslie

PY - 2014/9

Y1 - 2014/9

N2 - Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith-Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.

AB - Gram-negative bacterial outer membrane proteins play important roles in the interaction of bacteria with their environment including nutrient acquisition, adhesion and invasion, and antibiotic resistance. In this study we identified 47 proteins within the Sarkosyl-insoluble fraction of Campylobacter jejuni 81-176, using LC-ESI-MS/MS. Comparative analysis of outer membrane protein sequences was visualised to reveal protein distribution within a panel of Campylobacter spp., identifying several C. jejuni-specific proteins. Smith-Waterman analyses of C. jejuni homologues revealed high sequence conservation amongst a number of hypothetical proteins, sequence heterogeneity of other proteins and several proteins which are absent in a proportion of strains.

KW - Campylobacter jejuni

KW - Genomics

KW - Outer membrane

KW - Proteomics

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DO - 10.1016/j.euprot.2014.06.003

M3 - Article

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SN - 2212-9685

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EP - 194

JO - EuPA Open Proteomics

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ER -

Proteomic and genomic analysis reveals novel Campylobacter jejuni outer membrane proteins and potential heterogeneity

Abstract

Keywords

ASJC Scopus subject areas

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