Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Jason Brunt; Derek J. Jamieson; Brian Austin

doi:10.1016/j.vetimm.2007.09.010

Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Jason Brunt, Derek J. Jamieson, Brian Austin

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. © 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	199-205
Number of pages	7
Journal	Veterinary Immunology and Immunopathology
Volume	121
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetimm.2007.09.010
Publication status	Published - 15 Feb 2008

Keywords

Acute phase response
Fish disease
Innate immunity
Probiotic
Proteomics

Access to Document

10.1016/j.vetimm.2007.09.010

Cite this

@article{1783e41af17f4859be918c969157c618,

title = "Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets",

abstract = "The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. {\textcopyright} 2007 Elsevier B.V. All rights reserved.",

keywords = "Acute phase response, Fish disease, Innate immunity, Probiotic, Proteomics",

author = "Jason Brunt and Jamieson, \{Derek J.\} and Brian Austin",

year = "2008",

month = feb,

day = "15",

doi = "10.1016/j.vetimm.2007.09.010",

language = "English",

volume = "121",

pages = "199--205",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier B.V.",

number = "3-4",

}

TY - JOUR

T1 - Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

AU - Brunt, Jason

AU - Jamieson, Derek J.

AU - Austin, Brian

PY - 2008/2/15

Y1 - 2008/2/15

N2 - The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. © 2007 Elsevier B.V. All rights reserved.

AB - The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. © 2007 Elsevier B.V. All rights reserved.

KW - Acute phase response

KW - Fish disease

KW - Innate immunity

KW - Probiotic

KW - Proteomics

UR - https://www.scopus.com/pages/publications/38549173172

U2 - 10.1016/j.vetimm.2007.09.010

DO - 10.1016/j.vetimm.2007.09.010

M3 - Article

C2 - 17981340

SN - 0165-2427

VL - 121

SP - 199

EP - 205

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 3-4

ER -

Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this