Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Jason Brunt; Derek J. Jamieson; Brian Austin

doi:10.1016/j.vetimm.2007.09.010

Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Jason Brunt, Derek J. Jamieson, Brian Austin

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. © 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	199-205
Number of pages	7
Journal	Veterinary Immunology and Immunopathology
Volume	121
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetimm.2007.09.010
Publication status	Published - 15 Feb 2008

Keywords

Acute phase response
Fish disease
Innate immunity
Probiotic
Proteomics

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10.1016/j.vetimm.2007.09.010

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title = "Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets",

abstract = "The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. {\textcopyright} 2007 Elsevier B.V. All rights reserved.",

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AU - Jamieson, Derek J.

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AB - The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. © 2007 Elsevier B.V. All rights reserved.

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KW - Fish disease

KW - Innate immunity

KW - Probiotic

KW - Proteomics

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M3 - Article

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JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 3-4

ER -

Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Abstract

Keywords

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