Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Jason Brunt, Derek J. Jamieson, Brian Austin

    Research output: Contribution to journalArticle

    Abstract

    The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. © 2007 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)199-205
    Number of pages7
    JournalVeterinary Immunology and Immunopathology
    Volume121
    Issue number3-4
    DOIs
    Publication statusPublished - 15 Feb 2008

    Fingerprint

    Oncorhynchus mykiss
    Probiotics
    Proteomics
    Acute-Phase Reaction
    Diet
    Serum
    Aeromonas
    NADH Dehydrogenase
    Dystrophin
    Proteins
    Proteome
    Transferrin
    Bacillus
    Electrophoresis
    Mass Spectrometry

    Keywords

    • Acute phase response
    • Fish disease
    • Innate immunity
    • Probiotic
    • Proteomics

    Cite this

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    abstract = "The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. {\circledC} 2007 Elsevier B.V. All rights reserved.",
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    Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets. / Brunt, Jason; Jamieson, Derek J.; Austin, Brian.

    In: Veterinary Immunology and Immunopathology, Vol. 121, No. 3-4, 15.02.2008, p. 199-205.

    Research output: Contribution to journalArticle

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