Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets

Jason Brunt, Derek J. Jamieson, Brian Austin

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. © 2007 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)199-205
    Number of pages7
    JournalVeterinary Immunology and Immunopathology
    Volume121
    Issue number3-4
    DOIs
    Publication statusPublished - 15 Feb 2008

    Keywords

    • Acute phase response
    • Fish disease
    • Innate immunity
    • Probiotic
    • Proteomics

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