Proteomic analysis of Lawsonia intracellularis reveals expression of outer membrane proteins during infection

Eleanor Watson, M. Pilar Alberdi, Neil F. Inglis, Alex Lainson, Megan E. Porter, Erin Manson, Lisa Imrie, Kevin Mclean, David G E Smith

    Research output: Contribution to journalArticle

    Abstract

    Lawsonia intracellularis is the aetiological agent of the commercially significant porcine disease, proliferative enteropathy. Current understanding of host-pathogen interaction is limited due to the fastidious microaerophilic obligate intracellular nature of the bacterium. In the present study, expression of bacterial proteins during infection was investigated using a mass spectrometry approach. LC-ESI-MS/MS analysis of two isolates of L. intracellularis from heavily-infected epithelial cell cultures and database mining using fully annotated L. intracellularis genome sequences identified 19 proteins. According to the Clusters of Orthologous Groups (COG) functional classification, proteins were identified with roles in cell metabolism, protein synthesis and oxidative stress protection; seven proteins with putative or unknown function were also identified. Detailed bioinformatic analyses of five uncharacterised proteins, which were expressed by both isolates, identified domains and motifs common to other outer membrane-associated proteins with important roles in pathogenesis including adherence and invasion. Analysis of recombinant proteins on Western blots using immune sera from L. intracellularis-infected pigs identified two proteins, LI0841 and LI0902 as antigenic. The detection of five outer membrane proteins expressed during infection, including two antigenic proteins, demonstrates the potential of this approach to interrogate L. intracellularis host-pathogen interactions and identify novel targets which may be exploited in disease control.

    Original languageEnglish
    Pages (from-to)448-455
    Number of pages8
    JournalVeterinary Microbiology
    Volume174
    Issue number3-4
    DOIs
    Publication statusPublished - 5 Dec 2014

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    Watson, E., Alberdi, M. P., Inglis, N. F., Lainson, A., Porter, M. E., Manson, E., Imrie, L., Mclean, K., & Smith, D. G. E. (2014). Proteomic analysis of Lawsonia intracellularis reveals expression of outer membrane proteins during infection. Veterinary Microbiology, 174(3-4), 448-455. https://doi.org/10.1016/j.vetmic.2014.10.002