Proteomic analysis of a distilling strain of Saccharomyces cerevisiae during industrial grain fermentation

Rasmus Hansen, Stephen Y. Pearson, James M. Brosnan, Philip G. Meaden, Derek J. Jamieson

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    The fermentation performance of industrial yeast strains is influenced, among other things, by their genetic composition and the nature of the fermentable sugar, availability of nitrogen, and temperature. Therefore, to manipulate the fermentation process, it is important to understand, at a molecular level, the changes occurring in the yeast cell throughout industrial fermentation processes. With this aim in mind, using two-dimensional gel electrophoresis and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS), we have examined the proteome of distillers yeast in an industrial context. Using yeast sampled from a local grain whisky distillery, we have prepared a detailed reference map of the proteome of distillers yeast and have examined in some detail the alterations in protein levels that occur throughout fermentation. In particular, as fermentation progresses, there is a significant increase in the levels of a variety of proteins involved in protecting against stress and nitrogen limitation. These results therefore give an insight into the stresses that yeast are exposed to in industrial fermentations and reveal some of the proteins and enzymes that are either necessary or important for efficient fermentation. © Springer-Verlag 2006.

    Original languageEnglish
    Pages (from-to)116-125
    Number of pages10
    JournalApplied Microbiology and Biotechnology
    Volume72
    Issue number1
    DOIs
    Publication statusPublished - Aug 2006

    Keywords

    • Distillers yeast
    • Fermentation
    • Proteomics
    • Yeast

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