Proteolysis of SpoIVB is a critical determinant in signalling of Pro-σ(K) processing in Bacillus subtilis

Philip R. Wakeley, Robert Dorazi, Ngo Thi Hoa, John R. Bowyer, Simon M. Cutting

    Research output: Contribution to journalArticle

    Abstract

    SpoIVB is essential for intercompartmental signalling in the s(K)-checkpoint of Bacillus subtilis. SpoIVB is synthesized in the spore chamber and is the signal which activates proteolytic processing of pro-s(K) to its mature and active form s(K). We show here that SpoIVB is a serine peptidase of the SA clan. Expression of SpoIVB in Escherichia coli has shown that SpoIVB is able to self-cleave into at least three discrete products, and in vitro studies have shown cleavage in trans. Autoproteolysis of SpoIVB is tightly linked to the initiation of the two developmental functions of this protein, signalling of pro-s(K) processing and a yet, uncharacterized, second function which is essential for the formation of heat-resistant spores. In B. subtilis, SpoIVB is synthesized as a zymogen and is subject to two levels of proteolysis. First, autoproteolysis generating intermediate products, at least one of which is proposed to be the active form, followed by processing by one or more enzymes to smaller species. This could provide a mechanism for switching off the active SpoIVB intermediate(s) and suggests a similarity to other proteolytic cascades such as those found in blood coagulation.

    Original languageEnglish
    Pages (from-to)1336-1348
    Number of pages13
    JournalMolecular Microbiology
    Volume36
    Issue number6
    DOIs
    Publication statusPublished - 2000

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    Bacillus subtilis
    Spores
    Proteolysis
    Enzyme Precursors
    Blood Coagulation
    Serine
    Peptide Hydrolases
    Hot Temperature
    Escherichia coli
    Enzymes
    Proteins
    In Vitro Techniques

    Cite this

    Wakeley, Philip R. ; Dorazi, Robert ; Hoa, Ngo Thi ; Bowyer, John R. ; Cutting, Simon M. / Proteolysis of SpoIVB is a critical determinant in signalling of Pro-σ(K) processing in Bacillus subtilis. In: Molecular Microbiology. 2000 ; Vol. 36, No. 6. pp. 1336-1348.
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    abstract = "SpoIVB is essential for intercompartmental signalling in the s(K)-checkpoint of Bacillus subtilis. SpoIVB is synthesized in the spore chamber and is the signal which activates proteolytic processing of pro-s(K) to its mature and active form s(K). We show here that SpoIVB is a serine peptidase of the SA clan. Expression of SpoIVB in Escherichia coli has shown that SpoIVB is able to self-cleave into at least three discrete products, and in vitro studies have shown cleavage in trans. Autoproteolysis of SpoIVB is tightly linked to the initiation of the two developmental functions of this protein, signalling of pro-s(K) processing and a yet, uncharacterized, second function which is essential for the formation of heat-resistant spores. In B. subtilis, SpoIVB is synthesized as a zymogen and is subject to two levels of proteolysis. First, autoproteolysis generating intermediate products, at least one of which is proposed to be the active form, followed by processing by one or more enzymes to smaller species. This could provide a mechanism for switching off the active SpoIVB intermediate(s) and suggests a similarity to other proteolytic cascades such as those found in blood coagulation.",
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    Proteolysis of SpoIVB is a critical determinant in signalling of Pro-σ(K) processing in Bacillus subtilis. / Wakeley, Philip R.; Dorazi, Robert; Hoa, Ngo Thi; Bowyer, John R.; Cutting, Simon M.

    In: Molecular Microbiology, Vol. 36, No. 6, 2000, p. 1336-1348.

    Research output: Contribution to journalArticle

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