Proteins linked to drought tolerance revealed by DIGE analysis of drought resistant and susceptible barley varieties

Charlotte Wendelboe-Nelson, Peter C Morris

    Research output: Contribution to journalArticle

    Abstract

    Drought is a major threat to world agriculture. In order to identify proteins associated with plant drought tolerance, barley varieties bred in the UK (Golden Promise) and Iraq (Basrah) were compared. The variety Basrah showed physiological adaptations to drought when compared to Golden Promise, for example relative water content of roots and shoots after one week of drought was much higher for Basrah than for Golden Promise. DIGE analysis was carried out on proteins from roots and leaves under control and drought conditions. 24 leaf and 45 root proteins were identified by MALDI-TOF MS spectrometry. The relative expression patterns of the identified proteins fell into a number of distinct classes. The variety Basrah is characterised by constitutive expression or higher drought-induced expression levels of proteins regulating ROS production and protein folding. Photosynthetic enzymes, by contrast, were down-regulated in Basrah. Enzyme assays showed a good correlation between DIGE-derived protein abundance estimates and enzyme activity in extracts. Overall this study shows that the enhanced drought tolerance of variety Basrah is driven by an enhanced regulation of ROS under drought.
    Original languageEnglish
    Pages (from-to)3374-3385
    Number of pages11
    JournalProteomics
    Volume12
    Issue number22
    DOIs
    Publication statusPublished - 26 Nov 2012

    Fingerprint

    Droughts
    Hordeum
    Proteins
    Physiological Adaptation
    Iraq
    Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
    Protein Folding
    Enzyme Assays
    Enzymes
    Agriculture
    Spectrum Analysis
    Water

    Cite this

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    title = "Proteins linked to drought tolerance revealed by DIGE analysis of drought resistant and susceptible barley varieties",
    abstract = "Drought is a major threat to world agriculture. In order to identify proteins associated with plant drought tolerance, barley varieties bred in the UK (Golden Promise) and Iraq (Basrah) were compared. The variety Basrah showed physiological adaptations to drought when compared to Golden Promise, for example relative water content of roots and shoots after one week of drought was much higher for Basrah than for Golden Promise. DIGE analysis was carried out on proteins from roots and leaves under control and drought conditions. 24 leaf and 45 root proteins were identified by MALDI-TOF MS spectrometry. The relative expression patterns of the identified proteins fell into a number of distinct classes. The variety Basrah is characterised by constitutive expression or higher drought-induced expression levels of proteins regulating ROS production and protein folding. Photosynthetic enzymes, by contrast, were down-regulated in Basrah. Enzyme assays showed a good correlation between DIGE-derived protein abundance estimates and enzyme activity in extracts. Overall this study shows that the enhanced drought tolerance of variety Basrah is driven by an enhanced regulation of ROS under drought.",
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    Proteins linked to drought tolerance revealed by DIGE analysis of drought resistant and susceptible barley varieties. / Wendelboe-Nelson, Charlotte; Morris, Peter C.

    In: Proteomics, Vol. 12, No. 22, 26.11.2012, p. 3374-3385.

    Research output: Contribution to journalArticle

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    AU - Morris, Peter C

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