A ligno-cellulosic concentrate from municipal solid waste (MSW) obtained after an autoclave separation process was investigated for its potential as a feedstock to produce fermentable sugars for ethanol production. A maximum enzymatic hydrolysis conversion of 53% of the cellulose and hemi-cellulose was found using a particle size range of 150-300 mu m hydrolyzed in a 100 ml buffer solution containing 6 wt% lingo-cellulosic MSW concentrate with 90 mg cellulase at pH 4.8 held at 40 degrees C for 12 h. The hydrolysis rate leveled off at longer hydrolysis time and with increased substrate concentration and was related to enzymatic access to substrate. Lower hydrolysis rate at smaller particle size indicates that the grinding process may change the surface chemistry or morphology of the fibers making them less available for enzyme access. A drop in the hydrolysis rate was observed for the particles above 300 mu m associate with the longer diffusion time for the enzyme into the fiber particles. The findings indicate that 152 L of ethanol could be obtained from a ton of lingo-cellulosic concentrate from MSW. (C) 2011 Elsevier Ltd. All rights reserved.