Probing cell to cell variations in gene expression using microfluidics

Jung Uk Shim; Luis Olguin; Graeme Whyte; Duncan Scott; Florian Hollfelder; Chris Abell; Wilhelm Huck

Probing cell to cell variations in gene expression using microfluidics

Jung Uk Shim, Luis Olguin, Graeme Whyte, Duncan Scott, Florian Hollfelder, Chris Abell, Wilhelm Huck

University of Cambridge

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have developed a microfluidic system to investigate cell to cell variations in the expression level of a protein, Red Fluorescence Protein (mRFP1)[2], while cells have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for long term droplet storage[1]. We interrogate the expression level of mRFP1 by following the fluorescence of encapsulated cells with a microscope equipped with a motorized stage to monitor about 2000 stored droplets so that we can study how cells differently behave with respect to time in an identical environment.

Original language	English
Title of host publication	Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	1787-1789
Number of pages	3
ISBN (Print)	9780979806421
Publication status	Published - 2009
Event	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009

Conference

Conference	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2009
Abbreviated title	MicroTAS 2009
Country/Territory	Korea, Republic of
City	Jeju
Period	1/11/09 → 5/11/09

Keywords

Droplet
Heterogeneity
Microfluidics
Protein expression

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

@inproceedings{94dd3e2f0fb34ac1a6206c92110b2108,

title = "Probing cell to cell variations in gene expression using microfluidics",

abstract = "We have developed a microfluidic system to investigate cell to cell variations in the expression level of a protein, Red Fluorescence Protein (mRFP1)[2], while cells have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for long term droplet storage[1]. We interrogate the expression level of mRFP1 by following the fluorescence of encapsulated cells with a microscope equipped with a motorized stage to monitor about 2000 stored droplets so that we can study how cells differently behave with respect to time in an identical environment.",

keywords = "Droplet, Heterogeneity, Microfluidics, Protein expression",

author = "Shim, {Jung Uk} and Luis Olguin and Graeme Whyte and Duncan Scott and Florian Hollfelder and Chris Abell and Wilhelm Huck",

year = "2009",

language = "English",

isbn = "9780979806421",

pages = "1787--1789",

booktitle = "Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

note = "13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2009, MicroTAS 2009 ; Conference date: 01-11-2009 Through 05-11-2009",

}

Shim, JU, Olguin, L, Whyte, G, Scott, D, Hollfelder, F, Abell, C & Huck, W 2009, Probing cell to cell variations in gene expression using microfluidics. in Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1787-1789, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2009, Jeju, Korea, Republic of, 1/11/09.

Probing cell to cell variations in gene expression using microfluidics. / Shim, Jung Uk; Olguin, Luis; Whyte, Graeme et al.

Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1787-1789.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Probing cell to cell variations in gene expression using microfluidics

AU - Shim, Jung Uk

AU - Olguin, Luis

AU - Whyte, Graeme

AU - Scott, Duncan

AU - Hollfelder, Florian

AU - Abell, Chris

AU - Huck, Wilhelm

PY - 2009

Y1 - 2009

N2 - We have developed a microfluidic system to investigate cell to cell variations in the expression level of a protein, Red Fluorescence Protein (mRFP1)[2], while cells have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for long term droplet storage[1]. We interrogate the expression level of mRFP1 by following the fluorescence of encapsulated cells with a microscope equipped with a motorized stage to monitor about 2000 stored droplets so that we can study how cells differently behave with respect to time in an identical environment.

AB - We have developed a microfluidic system to investigate cell to cell variations in the expression level of a protein, Red Fluorescence Protein (mRFP1)[2], while cells have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for long term droplet storage[1]. We interrogate the expression level of mRFP1 by following the fluorescence of encapsulated cells with a microscope equipped with a motorized stage to monitor about 2000 stored droplets so that we can study how cells differently behave with respect to time in an identical environment.

KW - Droplet

KW - Heterogeneity

KW - Microfluidics

KW - Protein expression

UR - http://www.scopus.com/inward/record.url?scp=84901799274&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84901799274

SN - 9780979806421

SP - 1787

EP - 1789

BT - Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2009

Y2 - 1 November 2009 through 5 November 2009

ER -

Probing cell to cell variations in gene expression using microfluidics

Abstract

Conference

Keywords

ASJC Scopus subject areas

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Cite this