Probing cell to cell variations in gene expression using microfluidics

Jung Uk Shim, Luis Olguin, Graeme Whyte, Duncan Scott, Florian Hollfelder, Chris Abell, Wilhelm Huck

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a microfluidic system to investigate cell to cell variations in the expression level of a protein, Red Fluorescence Protein (mRFP1)[2], while cells have been encapsulated in microdroplets. The picoliter sized droplets are manipulated in a PDMS (polydimethylsiloxane) based microfluidic device specially designed for long term droplet storage[1]. We interrogate the expression level of mRFP1 by following the fluorescence of encapsulated cells with a microscope equipped with a motorized stage to monitor about 2000 stored droplets so that we can study how cells differently behave with respect to time in an identical environment.

Original languageEnglish
Title of host publicationProceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1787-1789
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2009
Abbreviated titleMicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Droplet
  • Heterogeneity
  • Microfluidics
  • Protein expression

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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