Preparation and utilization of a 3D human liver microtissue model for nanotoxicological assessment

Ali Kermanizadeh

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The liver is the principal detoxification center of the body, removing xenobiotics and waste products which could potentially include some nanomaterials (NM). With the ever increasing public and occupational exposure associated with accumulative production of nanomaterials, there is an urgent need to consider the possibility of harmful health consequences of engineered NM exposure. It is understood that following exposure via inhalation, ingestion, or direct intravenous injection a fraction of NMs reach the liver. Traditional in vitro or ex vivo hepatic nanotoxicology models are often limiting and/or troublesome (i.e., reduced metabolism enzymes, lacking important cell populations, unstable with very high variability, etc.). This chapter highlights a methodology for the preparation of a physiologically relevant 3D human liver microtissue model which addresses most of the negative issues associated with the models used in traditional in vitro hepatic toxicological investigations. The spheroids are a very promising model for the assessment of the toxicological effects associated with engineered NM exposure.

Original languageEnglish
Title of host publicationNanotoxicity
Subtitle of host publicationMethods and Protocols
EditorsQunwei Zhang
PublisherHumana Press
Pages47-55
Number of pages9
ISBN (Electronic)9781493989164
ISBN (Print)9781493989157
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1894
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • 3D microtissue spheroid
  • Hepatocytes
  • Kupffer cells
  • Liver
  • Nanomaterials
  • Nanotoxicology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kermanizadeh, A. (2019). Preparation and utilization of a 3D human liver microtissue model for nanotoxicological assessment. In Q. Zhang (Ed.), Nanotoxicity: Methods and Protocols (pp. 47-55). (Methods in Molecular Biology; Vol. 1894). Humana Press. https://doi.org/10.1007/978-1-4939-8916-4_3