Preparation and characterization of polyethylenimine-coated Fe3O4-MCM-48 nanocomposite particles as a novel agent for magnet-assisted transfection

Humphrey Hak Ping Yiu; Stuart McBain; Z. A D Lethbridge; M. R. Lees; Jon Dobson

doi:10.1002/jbm.a.32363

Preparation and characterization of polyethylenimine-coated Fe₃O₄-MCM-48 nanocomposite particles as a novel agent for magnet-assisted transfection

Humphrey Hak Ping Yiu, Stuart McBain, Z. A D Lethbridge, M. R. Lees, Jon Dobson

School of Engineering & Physical Sciences

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

A new type of magnetic nanoparticle was synthesized using mesoporous silica MCM-48 as a template. Magnetite (Fe(3)O(4)) nanocrystals were incorporated onto the MCM-48 silica structure by thermal decomposition of iron(Ill) acetylacetonate. The particle size of these Fe(3)O(4)-MCM-48 composite particles is around 300 nm with an iron oxide content of ca. 20% w/w. Measurements from SQUID magnetometry suggest that these nanoparticles possess superparamagnetic properties similar to those of Fe(3)O(4) nanoparticles. By coating positively charged polyethylenimine on to the surface, DNA can be bound onto the Fe(3)O(4)-MCM-48 nanoparticles. Transfection studies showed that these PEI-Fe(3)O(4)-MCM-48 particles were highly effective as a transfection reagent, and a 400% increase of transfection efficiency compared with the commercial products was recorded.

Original language	English
Pages (from-to)	386-392
Number of pages	7
Journal	Journal of Biomedical Materials Research Part A
Volume	92A
Issue number	1
DOIs	https://doi.org/10.1002/jbm.a.32363
Publication status	Published - Jan 2010

Keywords

gene delivery
polyethyleneimine
nanoporous
magnetite
magnetofection

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@article{35139d5b84fb43a69b9a6781d393bd30,

title = "Preparation and characterization of polyethylenimine-coated Fe3O4-MCM-48 nanocomposite particles as a novel agent for magnet-assisted transfection",

abstract = "A new type of magnetic nanoparticle was synthesized using mesoporous silica MCM-48 as a template. Magnetite (Fe(3)O(4)) nanocrystals were incorporated onto the MCM-48 silica structure by thermal decomposition of iron(Ill) acetylacetonate. The particle size of these Fe(3)O(4)-MCM-48 composite particles is around 300 nm with an iron oxide content of ca. 20% w/w. Measurements from SQUID magnetometry suggest that these nanoparticles possess superparamagnetic properties similar to those of Fe(3)O(4) nanoparticles. By coating positively charged polyethylenimine on to the surface, DNA can be bound onto the Fe(3)O(4)-MCM-48 nanoparticles. Transfection studies showed that these PEI-Fe(3)O(4)-MCM-48 particles were highly effective as a transfection reagent, and a 400% increase of transfection efficiency compared with the commercial products was recorded. ",

keywords = "gene delivery, polyethyleneimine, nanoporous, magnetite, magnetofection",

author = "Yiu, {Humphrey Hak Ping} and Stuart McBain and Lethbridge, {Z. A D} and Lees, {M. R.} and Jon Dobson",

year = "2010",

month = jan,

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language = "English",

volume = "92A",

pages = "386--392",

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issn = "1549-3296",

publisher = "John Wiley and Sons Inc.",

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Preparation and characterization of polyethylenimine-coated Fe₃O₄-MCM-48 nanocomposite particles as a novel agent for magnet-assisted transfection. / Yiu, Humphrey Hak Ping; McBain, Stuart; Lethbridge, Z. A D; Lees, M. R.; Dobson, Jon.

In: Journal of Biomedical Materials Research Part A, Vol. 92A, No. 1, 01.2010, p. 386-392.

Research output: Contribution to journal › Article › peer-review

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T1 - Preparation and characterization of polyethylenimine-coated Fe3O4-MCM-48 nanocomposite particles as a novel agent for magnet-assisted transfection

AU - Yiu, Humphrey Hak Ping

AU - McBain, Stuart

AU - Lethbridge, Z. A D

AU - Lees, M. R.

AU - Dobson, Jon

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AB - A new type of magnetic nanoparticle was synthesized using mesoporous silica MCM-48 as a template. Magnetite (Fe(3)O(4)) nanocrystals were incorporated onto the MCM-48 silica structure by thermal decomposition of iron(Ill) acetylacetonate. The particle size of these Fe(3)O(4)-MCM-48 composite particles is around 300 nm with an iron oxide content of ca. 20% w/w. Measurements from SQUID magnetometry suggest that these nanoparticles possess superparamagnetic properties similar to those of Fe(3)O(4) nanoparticles. By coating positively charged polyethylenimine on to the surface, DNA can be bound onto the Fe(3)O(4)-MCM-48 nanoparticles. Transfection studies showed that these PEI-Fe(3)O(4)-MCM-48 particles were highly effective as a transfection reagent, and a 400% increase of transfection efficiency compared with the commercial products was recorded.

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KW - polyethyleneimine

KW - nanoporous

KW - magnetite

KW - magnetofection

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