Possible effect of hala extract (Pandanus tectorius) on immune status, anti-tumour and resistance to Yersinia ruckeri infection in rainbow trout (Oncorhynchus mykiss)

Elham Awad, Dawn Austin, Alastair Lyndon, Amani Awaad

Research output: Contribution to journalArticle

9 Citations (Scopus)
6 Downloads (Pure)

Abstract

The possible effect of dietary administration of hala extract (Pandanus tectorius) on rainbow trout (Oncorhynchus mykiss) immune status as well as its effect as an anti-tumour agent was studied. Fish were divided into 4 groups before feeding with commercial diet (0%, control; 0.5%, 1% and 2% of hala extract) for 2 weeks. The effect of diet on the humoral immune parameters, ie total protein, myeloperoxidase content, antiproteases, lysozyme and bactericidal activities were studied. Also, the effect of the diets on the expression of some immune-related genes in rainbow trout head-kidney (TNF, LYZ2, IL-8 and CD-4) as well as tumour suppressor gene (WT-1a) was investigated. At the end of the feeding trial fish groups were challenged with Yersinia ruckeri. The results demonstrated enhancement in all the immune parameters in fish fed hala extract diets compared to control fish especially with the highest dose (2%) which recorded the highest significant increase (p < 0.05) in some parameters (total protein, myeloperoxidase content, antiproteases, and bactericidal activities) compared to the control. The results obtained from challenge with Y. ruckeri revealed reduction in the mortalities in fish groups fed with 1% and 2% doses of hala extract. Feeding with hala extract provoked upregulation in all immune- related genes. Again, the highest dose of hala extract showed a significant upregulation in WT1a expression (p < 0.05). The current study suggest that the hala extract, especially the highest dose, could be considered a good food additive to improve the immune status, resist tumour formation and to resist or control infectious diseases of rainbow trout.

Original languageEnglish
Pages (from-to)620-626
Number of pages7
JournalFish and Shellfish Immunology
Volume87
Early online date10 Feb 2019
DOIs
Publication statusPublished - Apr 2019

Keywords

  • Anti-tumour
  • Immune response
  • Pandanus tectorius
  • Rainbow trout
  • Yersinia ruckeri

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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