(Poly)phenol metabolites from Arbutus unedo leaves protect yeast from oxidative injury by activation of antioxidant and protein clearance pathways

Carolina Jardim; Diana Macedo; Inês Figueira; Gary Dobson; Gordon J. McDougall; Derek Stewart; Ricardo B. Ferreira; Regina Menezes; Cláudia N. Santos

doi:10.1016/j.jff.2017.03.010

(Poly)phenol metabolites from Arbutus unedo leaves protect yeast from oxidative injury by activation of antioxidant and protein clearance pathways

Carolina Jardim, Diana Macedo, Inês Figueira, Gary Dobson, Gordon J. McDougall, Derek Stewart, Ricardo B. Ferreira, Regina Menezes^*, Cláudia N. Santos

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Oxidative stress is a key process in several diseases and a growing body of preclinical/clinical evidence has pushed forward the health benefits of (poly)phenols. The protective potential of Corema album and Arbutus unedo (Poly)phenol Digested Metabolites (PDM), generated after in vitro digestion was evaluated. Chemical alterations caused by the digestion led to alterations of compounds reactivity. A. unedo leaf PDM arose as the fraction conferring the highest protective activity to the wild type and oxidative stress-sensitive yap1 yeast strains challenged with H₂O₂. Our mechanistic studies disclosed the effect of A. unedo leaf PDM on cell redox homeostasis and mitochondrial function, and identified HAP4, SOD1, SOD2, GSH1 and GLR1 as mediators of cellular protection. Also, RPN4- and ATG8-associated protein clearance pathways were revealed as putative targets. Overall, these data suggest the nutraceutical potential of A. unedo leaf PDM as a functional food for human health.

Original language	English
Pages (from-to)	333-346
Number of pages	14
Journal	Journal of Functional Foods
Volume	32
Early online date	14 Mar 2017
DOIs	https://doi.org/10.1016/j.jff.2017.03.010
Publication status	Published - May 2017

Keywords

Bioaccessibility
Oxidative stress
Polyphenols
Redox homoeostasis
Saccharomyces cerevisiae
Yap1

ASJC Scopus subject areas

Medicine (miscellaneous)
Food Science
Nutrition and Dietetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jff.2017.03.010

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title = "(Poly)phenol metabolites from Arbutus unedo leaves protect yeast from oxidative injury by activation of antioxidant and protein clearance pathways",

abstract = "Oxidative stress is a key process in several diseases and a growing body of preclinical/clinical evidence has pushed forward the health benefits of (poly)phenols. The protective potential of Corema album and Arbutus unedo (Poly)phenol Digested Metabolites (PDM), generated after in vitro digestion was evaluated. Chemical alterations caused by the digestion led to alterations of compounds reactivity. A. unedo leaf PDM arose as the fraction conferring the highest protective activity to the wild type and oxidative stress-sensitive yap1 yeast strains challenged with H2O2. Our mechanistic studies disclosed the effect of A. unedo leaf PDM on cell redox homeostasis and mitochondrial function, and identified HAP4, SOD1, SOD2, GSH1 and GLR1 as mediators of cellular protection. Also, RPN4- and ATG8-associated protein clearance pathways were revealed as putative targets. Overall, these data suggest the nutraceutical potential of A. unedo leaf PDM as a functional food for human health.",

keywords = "Bioaccessibility, Oxidative stress, Polyphenols, Redox homoeostasis, Saccharomyces cerevisiae, Yap1",

author = "Carolina Jardim and Diana Macedo and In{\^e}s Figueira and Gary Dobson and McDougall, {Gordon J.} and Derek Stewart and Ferreira, {Ricardo B.} and Regina Menezes and Santos, {Cl{\'a}udia N.}",

year = "2017",

month = may,

doi = "10.1016/j.jff.2017.03.010",

language = "English",

volume = "32",

pages = "333--346",

journal = "Journal of Functional Foods",

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T1 - (Poly)phenol metabolites from Arbutus unedo leaves protect yeast from oxidative injury by activation of antioxidant and protein clearance pathways

AU - Jardim, Carolina

AU - Macedo, Diana

AU - Figueira, Inês

AU - Dobson, Gary

AU - McDougall, Gordon J.

AU - Stewart, Derek

AU - Ferreira, Ricardo B.

AU - Menezes, Regina

AU - Santos, Cláudia N.

PY - 2017/5

Y1 - 2017/5

N2 - Oxidative stress is a key process in several diseases and a growing body of preclinical/clinical evidence has pushed forward the health benefits of (poly)phenols. The protective potential of Corema album and Arbutus unedo (Poly)phenol Digested Metabolites (PDM), generated after in vitro digestion was evaluated. Chemical alterations caused by the digestion led to alterations of compounds reactivity. A. unedo leaf PDM arose as the fraction conferring the highest protective activity to the wild type and oxidative stress-sensitive yap1 yeast strains challenged with H2O2. Our mechanistic studies disclosed the effect of A. unedo leaf PDM on cell redox homeostasis and mitochondrial function, and identified HAP4, SOD1, SOD2, GSH1 and GLR1 as mediators of cellular protection. Also, RPN4- and ATG8-associated protein clearance pathways were revealed as putative targets. Overall, these data suggest the nutraceutical potential of A. unedo leaf PDM as a functional food for human health.

AB - Oxidative stress is a key process in several diseases and a growing body of preclinical/clinical evidence has pushed forward the health benefits of (poly)phenols. The protective potential of Corema album and Arbutus unedo (Poly)phenol Digested Metabolites (PDM), generated after in vitro digestion was evaluated. Chemical alterations caused by the digestion led to alterations of compounds reactivity. A. unedo leaf PDM arose as the fraction conferring the highest protective activity to the wild type and oxidative stress-sensitive yap1 yeast strains challenged with H2O2. Our mechanistic studies disclosed the effect of A. unedo leaf PDM on cell redox homeostasis and mitochondrial function, and identified HAP4, SOD1, SOD2, GSH1 and GLR1 as mediators of cellular protection. Also, RPN4- and ATG8-associated protein clearance pathways were revealed as putative targets. Overall, these data suggest the nutraceutical potential of A. unedo leaf PDM as a functional food for human health.

KW - Bioaccessibility

KW - Oxidative stress

KW - Polyphenols

KW - Redox homoeostasis

KW - Saccharomyces cerevisiae

KW - Yap1

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DO - 10.1016/j.jff.2017.03.010

M3 - Article

AN - SCOPUS:85015044027

SN - 1756-4646

VL - 32

SP - 333

EP - 346

JO - Journal of Functional Foods

JF - Journal of Functional Foods

ER -

(Poly)phenol metabolites from Arbutus unedo leaves protect yeast from oxidative injury by activation of antioxidant and protein clearance pathways

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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