TY - JOUR
T1 - Plasmid Mediated mcr-1.1 Colistin-Resistance in Clinical Extraintestinal Escherichia coli Strains Isolated in Poland
AU - Majewski, Piotr
AU - Gutowska, Anna
AU - Smith, David G. E.
AU - Hauschild, Tomasz
AU - Majewska, Paulina
AU - Hryszko, Tomasz
AU - Gizycka, Dominika
AU - Kedra, Boguslaw
AU - Kochanowicz, Jan
AU - Glowiński, Jerzy
AU - Drewnowska, Justyna
AU - Swiecicka, Izabela
AU - Sacha, Pawel T.
AU - Wieczorek, Piotr
AU - Iwaniuk, Dominika
AU - Sulewska, Anetta
AU - Charkiewicz, Radoslaw
AU - Makarewicz, Katarzyna
AU - Zebrowska, Agnieszka
AU - Czaban, Slawomir
AU - Radziwon, Piotr
AU - Niklinski, Jacek
AU - Tryniszewska, Elzbieta A.
N1 - Funding Information:
This work was financed by the MINIATURA research project (2017/01/X/NZ6/01852 - National Science Center, Poland), and supported by the Medical University of Bialystok statutory subsidy (SUB/1/DN/19/005/2222).
Publisher Copyright:
Copyright © 2021 Majewski, Gutowska, Smith, Hauschild, Majewska, Hryszko, Gizycka, Kedra, Kochanowicz, Glowiński, Drewnowska, Swiecicka, Sacha, Wieczorek, Iwaniuk, Sulewska, Charkiewicz, Makarewicz, Zebrowska, Czaban, Radziwon, Niklinski and Tryniszewska.
PY - 2021/12/10
Y1 - 2021/12/10
N2 - Objectives: The growing incidence of multidrug-resistant (MDR) bacteria is an inexorable and fatal challenge in modern medicine. Colistin is a cationic polypeptide considered a “last-resort” antimicrobial for treating infections caused by MDR Gram-negative bacterial pathogens. Plasmid-borne mcr colistin resistance emerged recently, and could potentially lead to essentially untreatable infections, particularly in hospital and veterinary (livestock farming) settings. In this study, we sought to establish the molecular basis of colistin-resistance in six extraintestinal Escherichia coli strains. Methods: Molecular investigation of colistin-resistance was performed in six extraintestinal E. coli strains isolated from patients hospitalized in Medical University Hospital, Bialystok, Poland. Complete structures of bacterial chromosomes and plasmids were recovered with use of both short- and long-read sequencing technologies and Unicycler hybrid assembly. Moreover, an electrotransformation assay was performed in order to confirm IncX4 plasmid influence on colistin-resistance phenotype in clinical E. coli strains. Results: Here we report on the emergence of six mcr-1.1-producing extraintestinal E. coli isolates with a number of virulence factors. Mobile pEtN transferase-encoding gene, mcr-1.1, has been proved to be encoded within a type IV secretion system (T4SS)-containing 33.3 kbp IncX4 plasmid pMUB-MCR, next to the PAP2-like membrane-associated lipid phosphatase gene. Conclusion: IncX4 mcr-containing plasmids are reported as increasingly disseminated among E. coli isolates, making it an “epidemic” plasmid, responsible for (i) dissemination of colistin-resistance determinants between different E. coli clones, and (ii) circulation between environmental, industrial, and clinical settings. Great effort needs to be taken to avoid further dissemination of plasmid-mediated colistin resistance among clinically relevant Gram-negative bacterial pathogens.
AB - Objectives: The growing incidence of multidrug-resistant (MDR) bacteria is an inexorable and fatal challenge in modern medicine. Colistin is a cationic polypeptide considered a “last-resort” antimicrobial for treating infections caused by MDR Gram-negative bacterial pathogens. Plasmid-borne mcr colistin resistance emerged recently, and could potentially lead to essentially untreatable infections, particularly in hospital and veterinary (livestock farming) settings. In this study, we sought to establish the molecular basis of colistin-resistance in six extraintestinal Escherichia coli strains. Methods: Molecular investigation of colistin-resistance was performed in six extraintestinal E. coli strains isolated from patients hospitalized in Medical University Hospital, Bialystok, Poland. Complete structures of bacterial chromosomes and plasmids were recovered with use of both short- and long-read sequencing technologies and Unicycler hybrid assembly. Moreover, an electrotransformation assay was performed in order to confirm IncX4 plasmid influence on colistin-resistance phenotype in clinical E. coli strains. Results: Here we report on the emergence of six mcr-1.1-producing extraintestinal E. coli isolates with a number of virulence factors. Mobile pEtN transferase-encoding gene, mcr-1.1, has been proved to be encoded within a type IV secretion system (T4SS)-containing 33.3 kbp IncX4 plasmid pMUB-MCR, next to the PAP2-like membrane-associated lipid phosphatase gene. Conclusion: IncX4 mcr-containing plasmids are reported as increasingly disseminated among E. coli isolates, making it an “epidemic” plasmid, responsible for (i) dissemination of colistin-resistance determinants between different E. coli clones, and (ii) circulation between environmental, industrial, and clinical settings. Great effort needs to be taken to avoid further dissemination of plasmid-mediated colistin resistance among clinically relevant Gram-negative bacterial pathogens.
KW - colistin-resistance
KW - extraintestinal E. coli
KW - IncX4 plasmid
KW - mcr-1.1
KW - plasmid
UR - http://www.scopus.com/inward/record.url?scp=85121759051&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2021.547020
DO - 10.3389/fmicb.2021.547020
M3 - Article
C2 - 34956105
AN - SCOPUS:85121759051
SN - 1664-302X
VL - 12
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 547020
ER -