Phylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains

M. Aquino De Muro, F. G. Priest

    Research output: Contribution to journalArticle

    Abstract

    The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus species. It can be used to identify potential mosquito-pathogenic strains of B. sphaericus in screening programmes.

    Original languageEnglish
    Pages (from-to)205-210
    Number of pages6
    JournalFEMS Microbiology Letters
    Volume112
    Issue number2
    DOIs
    Publication statusPublished - 1993

    Fingerprint

    Oligonucleotide Probes
    Culicidae
    Bacillus
    rRNA Genes
    DNA
    Escherichia coli

    Keywords

    • Bacillus sphaericus
    • mosquito-toxic strains
    • oligonucleotide probe

    Cite this

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    abstract = "The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus species. It can be used to identify potential mosquito-pathogenic strains of B. sphaericus in screening programmes.",
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    Phylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains. / Aquino De Muro, M.; Priest, F. G.

    In: FEMS Microbiology Letters, Vol. 112, No. 2, 1993, p. 205-210.

    Research output: Contribution to journalArticle

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