Phylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains

M. Aquino De Muro; F. G. Priest

doi:10.1016/0378-1097(93)90165-X

Phylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains

M. Aquino De Muro, F. G. Priest

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus species. It can be used to identify potential mosquito-pathogenic strains of B. sphaericus in screening programmes.

Original language	English
Pages (from-to)	205-210
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	112
Issue number	2
DOIs	https://doi.org/10.1016/0378-1097(93)90165-X
Publication status	Published - 1993

Keywords

Bacillus sphaericus
mosquito-toxic strains
oligonucleotide probe

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10.1016/0378-1097(93)90165-X

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title = "Phylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains",

abstract = "The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus species. It can be used to identify potential mosquito-pathogenic strains of B. sphaericus in screening programmes.",

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AU - Aquino De Muro, M.

AU - Priest, F. G.

PY - 1993

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N2 - The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus species. It can be used to identify potential mosquito-pathogenic strains of B. sphaericus in screening programmes.

AB - The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology group IIA which contains all known mosquito pathogens and not to any of ten other Bacillus species. It can be used to identify potential mosquito-pathogenic strains of B. sphaericus in screening programmes.

KW - Bacillus sphaericus

KW - mosquito-toxic strains

KW - oligonucleotide probe

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U2 - 10.1016/0378-1097(93)90165-X

DO - 10.1016/0378-1097(93)90165-X

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VL - 112

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EP - 210

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Phylogenetic analysis of Bacillus sphaericus and development of an oligonucleotide probe specific for mosquito-pathogenic strains

Abstract

Keywords

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