PclA, a pneumococcal collagen-like protein with selected strain distribution, contributes to adherence and invasion of host cells

Gavin K. Paterson, Leena Nieminen, Johanna M. C. Jefferies, Tim J. Mitchell

Research output: Contribution to journalArticle

Abstract

Analysis of Streptococcus pneumoniae sequenced genomes revealed a region present only in selected strains consisting of two ORFs: a putative cell wall anchored protein and a putative transcriptional regulator. The cell wall anchored protein contains large regions of collagen-like repeats, the number of which varies between strains. We have therefore named this protein PclA for pneumococcal collagen-like protein A. The second gene, spr1404, encodes a putative transcriptional regulator. We examined the strain distribution of these two genes among a collection of clinical isolates from invasive pneumococcal disease and found them to be present in 39% of the strains examined. Strains were either positive for both genes or lacked both, with the two genes always present together in the same location of the genome. RT-PCR analysis revealed that pclA is transcribed in vitro, even in the absence of spr1404. Single deletion mutants lacking either gene were not attenuated in a mouse model of invasive pneumonia. However, the pclA mutant was defective in adherence and invasion of host cells in vitro.

Original languageEnglish
Pages (from-to)170-176
Number of pages7
JournalFEMS Microbiology Letters
Volume285
Issue number2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Collagen
Genes
Proteins
Cell Wall
Genome
Staphylococcal Protein A
Streptococcus pneumoniae
Open Reading Frames
Pneumonia
Polymerase Chain Reaction
In Vitro Techniques

Keywords

  • Adhesins, Bacterial/genetics
  • Amino Acid Motifs
  • Animals
  • Bacterial Adhesion
  • Blood/microbiology
  • DNA, Bacterial/genetics
  • Female
  • Gene Deletion
  • Gene Expression Profiling
  • Gene Order
  • Genes, Bacterial
  • Mice
  • Pneumonia, Pneumococcal/microbiology
  • Polymerase Chain Reaction
  • RNA, Bacterial/biosynthesis
  • RNA, Messenger/biosynthesis
  • Repetitive Sequences, Amino Acid
  • Reverse Transcriptase Polymerase Chain Reaction
  • Streptococcus pneumoniae/physiology
  • Survival Analysis
  • Transcription Factors/genetics
  • Virulence
  • Virulence Factors/genetics

Cite this

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abstract = "Analysis of Streptococcus pneumoniae sequenced genomes revealed a region present only in selected strains consisting of two ORFs: a putative cell wall anchored protein and a putative transcriptional regulator. The cell wall anchored protein contains large regions of collagen-like repeats, the number of which varies between strains. We have therefore named this protein PclA for pneumococcal collagen-like protein A. The second gene, spr1404, encodes a putative transcriptional regulator. We examined the strain distribution of these two genes among a collection of clinical isolates from invasive pneumococcal disease and found them to be present in 39{\%} of the strains examined. Strains were either positive for both genes or lacked both, with the two genes always present together in the same location of the genome. RT-PCR analysis revealed that pclA is transcribed in vitro, even in the absence of spr1404. Single deletion mutants lacking either gene were not attenuated in a mouse model of invasive pneumonia. However, the pclA mutant was defective in adherence and invasion of host cells in vitro.",
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PclA, a pneumococcal collagen-like protein with selected strain distribution, contributes to adherence and invasion of host cells. / Paterson, Gavin K.; Nieminen, Leena; Jefferies, Johanna M. C.; Mitchell, Tim J.

In: FEMS Microbiology Letters, Vol. 285, No. 2, 08.2008, p. 170-176.

Research output: Contribution to journalArticle

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AU - Mitchell, Tim J.

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