Abstract
Analysis of Streptococcus pneumoniae sequenced genomes revealed a region present only in selected strains consisting of two ORFs: a putative cell wall anchored protein and a putative transcriptional regulator. The cell wall anchored protein contains large regions of collagen-like repeats, the number of which varies between strains. We have therefore named this protein PclA for pneumococcal collagen-like protein A. The second gene, spr1404, encodes a putative transcriptional regulator. We examined the strain distribution of these two genes among a collection of clinical isolates from invasive pneumococcal disease and found them to be present in 39% of the strains examined. Strains were either positive for both genes or lacked both, with the two genes always present together in the same location of the genome. RT-PCR analysis revealed that pclA is transcribed in vitro, even in the absence of spr1404. Single deletion mutants lacking either gene were not attenuated in a mouse model of invasive pneumonia. However, the pclA mutant was defective in adherence and invasion of host cells in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 170-176 |
| Number of pages | 7 |
| Journal | FEMS Microbiology Letters |
| Volume | 285 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2008 |
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Keywords
- Adhesins, Bacterial/genetics
- Amino Acid Motifs
- Animals
- Bacterial Adhesion
- Blood/microbiology
- DNA, Bacterial/genetics
- Female
- Gene Deletion
- Gene Expression Profiling
- Gene Order
- Genes, Bacterial
- Mice
- Pneumonia, Pneumococcal/microbiology
- Polymerase Chain Reaction
- RNA, Bacterial/biosynthesis
- RNA, Messenger/biosynthesis
- Repetitive Sequences, Amino Acid
- Reverse Transcriptase Polymerase Chain Reaction
- Streptococcus pneumoniae/physiology
- Survival Analysis
- Transcription Factors/genetics
- Virulence
- Virulence Factors/genetics
Cite this
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PclA, a pneumococcal collagen-like protein with selected strain distribution, contributes to adherence and invasion of host cells. / Paterson, Gavin K.; Nieminen, Leena; Jefferies, Johanna M. C.; Mitchell, Tim J.
In: FEMS Microbiology Letters, Vol. 285, No. 2, 08.2008, p. 170-176.Research output: Contribution to journal › Article
TY - JOUR
T1 - PclA, a pneumococcal collagen-like protein with selected strain distribution, contributes to adherence and invasion of host cells
AU - Paterson, Gavin K.
AU - Nieminen, Leena
AU - Jefferies, Johanna M. C.
AU - Mitchell, Tim J.
PY - 2008/8
Y1 - 2008/8
N2 - Analysis of Streptococcus pneumoniae sequenced genomes revealed a region present only in selected strains consisting of two ORFs: a putative cell wall anchored protein and a putative transcriptional regulator. The cell wall anchored protein contains large regions of collagen-like repeats, the number of which varies between strains. We have therefore named this protein PclA for pneumococcal collagen-like protein A. The second gene, spr1404, encodes a putative transcriptional regulator. We examined the strain distribution of these two genes among a collection of clinical isolates from invasive pneumococcal disease and found them to be present in 39% of the strains examined. Strains were either positive for both genes or lacked both, with the two genes always present together in the same location of the genome. RT-PCR analysis revealed that pclA is transcribed in vitro, even in the absence of spr1404. Single deletion mutants lacking either gene were not attenuated in a mouse model of invasive pneumonia. However, the pclA mutant was defective in adherence and invasion of host cells in vitro.
AB - Analysis of Streptococcus pneumoniae sequenced genomes revealed a region present only in selected strains consisting of two ORFs: a putative cell wall anchored protein and a putative transcriptional regulator. The cell wall anchored protein contains large regions of collagen-like repeats, the number of which varies between strains. We have therefore named this protein PclA for pneumococcal collagen-like protein A. The second gene, spr1404, encodes a putative transcriptional regulator. We examined the strain distribution of these two genes among a collection of clinical isolates from invasive pneumococcal disease and found them to be present in 39% of the strains examined. Strains were either positive for both genes or lacked both, with the two genes always present together in the same location of the genome. RT-PCR analysis revealed that pclA is transcribed in vitro, even in the absence of spr1404. Single deletion mutants lacking either gene were not attenuated in a mouse model of invasive pneumonia. However, the pclA mutant was defective in adherence and invasion of host cells in vitro.
KW - Adhesins, Bacterial/genetics
KW - Amino Acid Motifs
KW - Animals
KW - Bacterial Adhesion
KW - Blood/microbiology
KW - DNA, Bacterial/genetics
KW - Female
KW - Gene Deletion
KW - Gene Expression Profiling
KW - Gene Order
KW - Genes, Bacterial
KW - Mice
KW - Pneumonia, Pneumococcal/microbiology
KW - Polymerase Chain Reaction
KW - RNA, Bacterial/biosynthesis
KW - RNA, Messenger/biosynthesis
KW - Repetitive Sequences, Amino Acid
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Streptococcus pneumoniae/physiology
KW - Survival Analysis
KW - Transcription Factors/genetics
KW - Virulence
KW - Virulence Factors/genetics
U2 - 10.1111/j.1574-6968.2008.01217.x
DO - 10.1111/j.1574-6968.2008.01217.x
M3 - Article
VL - 285
SP - 170
EP - 176
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -