Partial Purification and Properties of a Bacterial Isoamylase

Richard M Evans, David J Manners, J Roger Stark

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    22 Citations (Scopus)

    Abstract

    Isoamylase has been prepared by affinity chromatography of a commercial enzyme-preparation from a strain of Cytophaga (also known as a Flavobacterium] or Polyangium). The enzyme was not very stable, but the stability could be improved by calcium ions. The enzyme had a very low but significant activity on pullulan and on alpha-dextrins having maltosyl side-chains. This observation, which is contrary to previous reports, has been related to the specificity of isoamylase and other barterial debranching-enzymes. © 1979.

    Original languageEnglish
    Pages (from-to)203-213
    Number of pages11
    JournalCarbohydrate Research
    Volume76
    Issue number1
    Publication statusPublished - Nov 1979

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