Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production

stress biomarker or oxidative stress signalling molecule?

L. Foucaud, S. Goulaouic, A. Bennasroune, P. Laval-Gilly, D. Brown, V. Stone, J. Falla

    Research output: Contribution to journalArticle

    Abstract

    The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of 4-HNE in inducing further cytotoxic effects.

    ROS production in cells by CB nanoparticles was shown by the oxidation of DCFH after a short time exposure. These particles induced the formation of 4-HNE-protein adducts and significant modification of glutathione content corresponding to an increase of oxidized glutathione form (GSSG) and a decrease of total glutathione (GSX) content. These results attest to an oxidative stress induced by the carbon black nanoparticles, although no induction of HO-1 protein expression was detected. Concerning the effects of a direct exposure to 4-HNE, our results showed that 4-HNE is not cytotoxic for concentrations lower than 12.5 mu M. By contrast, it provokes a very high cytotoxicity for concentrations above 25 mu M. An induction of HO-1 expression was observed from concentrations above 5 mu M of 4-HNE. Finally, glutathione content decreased significantly from 5 mu M of 4-HNE but no modification was observed under this concentration.

    The discrepancy between effects of carbon black nanoparticles and 4-HNE on the intracellular markers of oxidative stress suggests that 4-HNE is not directly implied in the signalling of oxidative toxicity of nanoparticles but is an effective biomarker of oxidative effects of nanoparticles. (C) 2010 Elsevier Ltd. All rights reserved.

    Original languageEnglish
    Pages (from-to)1512-1520
    Number of pages9
    JournalToxicology in Vitro
    Volume24
    Issue number6
    DOIs
    Publication statusPublished - Sep 2010

    Cite this

    Foucaud, L. ; Goulaouic, S. ; Bennasroune, A. ; Laval-Gilly, P. ; Brown, D. ; Stone, V. ; Falla, J. / Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production : stress biomarker or oxidative stress signalling molecule?. In: Toxicology in Vitro. 2010 ; Vol. 24, No. 6. pp. 1512-1520.
    @article{ce202c83b1c0477c91aa8a076ee6f088,
    title = "Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production: stress biomarker or oxidative stress signalling molecule?",
    abstract = "The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of 4-HNE in inducing further cytotoxic effects.ROS production in cells by CB nanoparticles was shown by the oxidation of DCFH after a short time exposure. These particles induced the formation of 4-HNE-protein adducts and significant modification of glutathione content corresponding to an increase of oxidized glutathione form (GSSG) and a decrease of total glutathione (GSX) content. These results attest to an oxidative stress induced by the carbon black nanoparticles, although no induction of HO-1 protein expression was detected. Concerning the effects of a direct exposure to 4-HNE, our results showed that 4-HNE is not cytotoxic for concentrations lower than 12.5 mu M. By contrast, it provokes a very high cytotoxicity for concentrations above 25 mu M. An induction of HO-1 expression was observed from concentrations above 5 mu M of 4-HNE. Finally, glutathione content decreased significantly from 5 mu M of 4-HNE but no modification was observed under this concentration.The discrepancy between effects of carbon black nanoparticles and 4-HNE on the intracellular markers of oxidative stress suggests that 4-HNE is not directly implied in the signalling of oxidative toxicity of nanoparticles but is an effective biomarker of oxidative effects of nanoparticles. (C) 2010 Elsevier Ltd. All rights reserved.",
    author = "L. Foucaud and S. Goulaouic and A. Bennasroune and P. Laval-Gilly and D. Brown and V. Stone and J. Falla",
    year = "2010",
    month = "9",
    doi = "10.1016/j.tiv.2010.07.012",
    language = "English",
    volume = "24",
    pages = "1512--1520",
    journal = "Toxicology in Vitro",
    issn = "0887-2333",
    publisher = "Elsevier Limited",
    number = "6",

    }

    Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production : stress biomarker or oxidative stress signalling molecule? / Foucaud, L.; Goulaouic, S.; Bennasroune, A.; Laval-Gilly, P.; Brown, D.; Stone, V.; Falla, J.

    In: Toxicology in Vitro, Vol. 24, No. 6, 09.2010, p. 1512-1520.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Oxidative stress induction by nanoparticles in THP-1 cells with 4-HNE production

    T2 - stress biomarker or oxidative stress signalling molecule?

    AU - Foucaud, L.

    AU - Goulaouic, S.

    AU - Bennasroune, A.

    AU - Laval-Gilly, P.

    AU - Brown, D.

    AU - Stone, V.

    AU - Falla, J.

    PY - 2010/9

    Y1 - 2010/9

    N2 - The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of 4-HNE in inducing further cytotoxic effects.ROS production in cells by CB nanoparticles was shown by the oxidation of DCFH after a short time exposure. These particles induced the formation of 4-HNE-protein adducts and significant modification of glutathione content corresponding to an increase of oxidized glutathione form (GSSG) and a decrease of total glutathione (GSX) content. These results attest to an oxidative stress induced by the carbon black nanoparticles, although no induction of HO-1 protein expression was detected. Concerning the effects of a direct exposure to 4-HNE, our results showed that 4-HNE is not cytotoxic for concentrations lower than 12.5 mu M. By contrast, it provokes a very high cytotoxicity for concentrations above 25 mu M. An induction of HO-1 expression was observed from concentrations above 5 mu M of 4-HNE. Finally, glutathione content decreased significantly from 5 mu M of 4-HNE but no modification was observed under this concentration.The discrepancy between effects of carbon black nanoparticles and 4-HNE on the intracellular markers of oxidative stress suggests that 4-HNE is not directly implied in the signalling of oxidative toxicity of nanoparticles but is an effective biomarker of oxidative effects of nanoparticles. (C) 2010 Elsevier Ltd. All rights reserved.

    AB - The aim of this study was to investigate whether carbon black (CB) nanoparticles might induce toxicity to monocytic cells in vitro via an oxidative stress mechanism involving formation of the lipid peroxidation product 4-hydroxynonenal (4-HNE) and the subsequent role of 4-HNE in inducing further cytotoxic effects.ROS production in cells by CB nanoparticles was shown by the oxidation of DCFH after a short time exposure. These particles induced the formation of 4-HNE-protein adducts and significant modification of glutathione content corresponding to an increase of oxidized glutathione form (GSSG) and a decrease of total glutathione (GSX) content. These results attest to an oxidative stress induced by the carbon black nanoparticles, although no induction of HO-1 protein expression was detected. Concerning the effects of a direct exposure to 4-HNE, our results showed that 4-HNE is not cytotoxic for concentrations lower than 12.5 mu M. By contrast, it provokes a very high cytotoxicity for concentrations above 25 mu M. An induction of HO-1 expression was observed from concentrations above 5 mu M of 4-HNE. Finally, glutathione content decreased significantly from 5 mu M of 4-HNE but no modification was observed under this concentration.The discrepancy between effects of carbon black nanoparticles and 4-HNE on the intracellular markers of oxidative stress suggests that 4-HNE is not directly implied in the signalling of oxidative toxicity of nanoparticles but is an effective biomarker of oxidative effects of nanoparticles. (C) 2010 Elsevier Ltd. All rights reserved.

    U2 - 10.1016/j.tiv.2010.07.012

    DO - 10.1016/j.tiv.2010.07.012

    M3 - Article

    VL - 24

    SP - 1512

    EP - 1520

    JO - Toxicology in Vitro

    JF - Toxicology in Vitro

    SN - 0887-2333

    IS - 6

    ER -